Academic Journal
Activation induced cytidine deaminase mutant (AID-His130Pro) from Hyper IgM 2 patient retained mutagenic activity on SHM artificial substrate
| العنوان: | Activation induced cytidine deaminase mutant (AID-His130Pro) from Hyper IgM 2 patient retained mutagenic activity on SHM artificial substrate |
|---|---|
| المؤلفون: | Ouadani, Hanen, Benmustapha, Imen, Ben-Ali, Meriem, Larguèche, Beya, Jovanic, Tihana, Garcia, Sylvie, Arcangioli, Benoît, Elloumi-Zghal, Houda, Fathallah, Dahmani M, Hachicha, Mongia T., Masmoudi, Hatem, Rougeon, François, Barbouche, Mohamed-Ridha |
| المساهمون: | Laboratoire de Transmission, Contrôle et Immunobiologie des Infections - Laboratory of Transmission, Control and Immunobiology of Infection (LR11IPT02), Institut Pasteur de Tunis, Pasteur Network (Réseau International des Instituts Pasteur)-Pasteur Network (Réseau International des Instituts Pasteur), Université de Tunis El Manar (UTM), Génétique et Biochimie du Développement, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), Biologie des Populations Lymphocytaires, Dynamique du Génome - Dynamics of the genome, Hedi Chaker Hospital Sfax (CHU Sfax), This work was supported by the Ministry of Health (Tunisia). |
| المصدر: | ISSN: 0161-5890 ; Molecular Immunology ; https://riip.hal.science/pasteur-01453058 ; Molecular Immunology, 2016, 79, pp.77 - 82. ⟨10.1016/j.molimm.2016.09.025⟩. |
| بيانات النشر: | CCSD Elsevier |
| سنة النشر: | 2016 |
| المجموعة: | Réseau International des Instituts Pasteur, Paris: HAL-RIIP |
| مصطلحات موضوعية: | AID mutagenic activity, Activation induced cytidine deaminase, Class switch recombination, Cofactors, Hyper IgM 2, Somatic hypermutation, MESH: Blotting, Western, MESH: Cytidine Deaminase, MESH: Humans, MESH: Hyper-IgM Immunodeficiency Syndrome, MESH: Immunoglobulin Class Switching, MESH: Jurkat Cells, MESH: Mutagenesis, Site-Directed, MESH: Mutation, MESH: Somatic Hypermutation, Immunoglobulin, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Activation induced cytidine deaminase (AID) is an essential enzyme for class switch recombination (CSR) and somatic hypermutation (SHM) during secondary immune response. Mutations in the AICDA gene are responsible for Hyper IgM 2 syndrome where both CSR and SHM or only CSR are affected. Indeed, triggering either of the two mechanisms requires the DNA deamination activity of AID. Besides, different domains of AID may be differentially involved in CSR and SHM through their interaction with specific cofactors. Herein, we studied the AID-induced SHM activity of the AID-His130Pro mutant identified in a patient with Hyper IgM 2 syndrome. AID mutagenic activity was monitored by the reversion of nonsense mutations of the EGFP gene assessed by flow cytometry. We found that the His130Pro mutation, which affects CSR, preserves AID mutagenic activity. Indeed, the His130 residue is located in a putative specific CSR region in the APOBEC-like domain, known to involve CSR specific cofactors that probably play a major role in AID physiological activities. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/27716525; PUBMED: 27716525 |
| DOI: | 10.1016/j.molimm.2016.09.025 |
| الاتاحة: | https://riip.hal.science/pasteur-01453058 https://riip.hal.science/pasteur-01453058v1/document https://riip.hal.science/pasteur-01453058v1/file/Manuscript%20Hanen.pdf https://doi.org/10.1016/j.molimm.2016.09.025 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.1A7FBA42 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.molimm.2016.09.025 |
|---|