Academic Journal
Aggregation Of Chlorophyll a Probed By Resonance Light Scattering Spectroscopy
| العنوان: | Aggregation Of Chlorophyll a Probed By Resonance Light Scattering Spectroscopy |
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| المؤلفون: | de Paula, J. C., Robblee, J. H., Pasternack, Robert F. |
| المصدر: | Chemistry & Biochemistry Faculty Works |
| بيانات النشر: | Works |
| سنة النشر: | 1995 |
| المجموعة: | Swarthmore College: Works |
| مصطلحات موضوعية: | Inorganic Chemistry |
| الوصف: | We report the resonance light scattering (RLS) spectra of chlorophyll a aggregated in a 9:1 solution of formamide and pH 6.8 phosphate buffer. The aggregate formed after 2 h of mixing, referred to as Chl(469), shows a strong scattering feature at 469 nm (Soret band) and a much weaker feature at 699 nm (Q(y) band). A kinetic investigation confirmed that the aggregation process is cooperative, and also detected one intermediate (Chl(458)) with a strong RLS spectrum but only a weak CD spectrum. We propose that aggregation proceeds via at least three steps: 1) formation of a nucleating species, probably a dimer of chlorophylls; 2) formation of large aggregates with little or no secondary structure (e.g., Chl(458)); and 3) conformational change to form helical aggregate (Chl(469)). |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| Relation: | https://works.swarthmore.edu/fac-chemistry/109; https://works.swarthmore.edu/context/fac-chemistry/article/1108/viewcontent/1_s2.0_S000634959580192X_main.pdf |
| DOI: | 10.1016/s0006-3495(95)80192-x |
| الاتاحة: | https://works.swarthmore.edu/fac-chemistry/109 https://doi.org/10.1016/s0006-3495(95)80192-x https://works.swarthmore.edu/context/fac-chemistry/article/1108/viewcontent/1_s2.0_S000634959580192X_main.pdf |
| رقم الانضمام: | edsbas.1A6CA496 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/s0006-3495(95)80192-x |
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