Academic Journal
v-erbA overexpression is required to extinguish c-erbA function in erythroid cell differentiation and regulation of the erbA target gene CAII.
| العنوان: | v-erbA overexpression is required to extinguish c-erbA function in erythroid cell differentiation and regulation of the erbA target gene CAII. |
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| المؤلفون: | Disela, C, Glineur, C, Bugge, T, Sap, J, Stengl, G, Dodgson, J, Stunnenberg, H, Beug, H, Zenke, M |
| بيانات النشر: | Cold Spring Harbor Laboratory Press |
| سنة النشر: | 1991 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Research Papers |
| الوصف: | The v-erbA oncoprotein represents a retrovirus-transduced oncogenic version of the thyroid hormone (T3/T4) receptor c-erbA (type alpha). It contributes to virus-induced erythroleukemia by efficiently arresting differentiation of red cell progenitors and by suppressing transcription of erythrocyte-specific genes. Here, we show that v-erbA and c-erbA bind directly to sequences within the promoter of the erythrocyte-specific carbonic anhydrase II (CAII), a gene whose transcription is efficiently suppressed by v-erbA. This erbA-binding site confers thyroid hormone responsiveness to a heterologous promoter in transient expression experiments and is a target for efficient down-regulation of CAII transcription by the v-erbA oncoprotein. In stably transformed erythroblasts coexpressing the v-erbA oncoprotein and the c-erbA/T3 receptor at an approximately equimolar ratio, c-erbA activity is dominant over v-erbA. T3 efficiently induced erythroid differentiation in these cells, thus overcoming the v-erbA-mediated differentiation arrest. Likewise, T3 activated CAII transcription as well as transient expression of a T3-responsive reporter gene containing the CAII-specific erbA-binding site. The c-erbA-dependent activation of this CAII reporter construct could only be suppressed by very high amounts of v-erbA. Our results suggest that overexpression of v-erbA is required for its function as an oncoprotein. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| Relation: | http://genesdev.cshlp.org/cgi/content/short/5/11/2033; http://dx.doi.org/10.1101/gad.5.11.2033 |
| DOI: | 10.1101/gad.5.11.2033 |
| الاتاحة: | http://genesdev.cshlp.org/cgi/content/short/5/11/2033 https://doi.org/10.1101/gad.5.11.2033 |
| Rights: | Copyright (C) 1991, Cold Spring Harbor Laboratory Press |
| رقم الانضمام: | edsbas.1A6C1159 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1101/gad.5.11.2033 |
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