Academic Journal
A novel R3 MYB transcriptional repressor associated with the loss of floral pigmentation in Iochroma
| العنوان: | A novel R3 MYB transcriptional repressor associated with the loss of floral pigmentation in Iochroma |
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| المؤلفون: | Gates, Daniel J., Olson, Bradley J. S. C., Clemente, Tom E., Smith, Stacey D. |
| المساهمون: | National Institutes of Health, National Science Foundation, University of Nebraska-Lincoln |
| المصدر: | New Phytologist ; volume 217, issue 3, page 1346-1356 ; ISSN 0028-646X 1469-8137 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2017 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Summary Losses of floral pigmentation represent one of the most common evolutionary transitions in flower color, yet the genetic basis for these changes has been elucidated in only a handful of cases. Here we used crossing studies, bulk‐segregant RNA sequencing, phylogenetic analyses and functional tests to identify the gene(s) responsible for the transition to white flowers in Iochroma loxense . Crosses between I. loxense and its blue‐flowered sister species, I. cyaneum , suggested that a single locus controls the flower color difference and that the white allele causes a nearly complete loss of pigmentation. Examining sequence variation across phenotypic pools from the crosses, we found that alleles at a novel R3 MYB transcription factor were tightly associated with flower color variation. This gene, which we term MYBL 1 , falls into a class of MYB transcriptional repressors and, accordingly, higher expression of this gene is associated with downregulation of multiple anthocyanin pigment pathway genes. We confirmed the repressive function of MYBL 1 through stable transformation of Nicotiana . The mechanism underlying the evolution of white flowers in I. loxense differs from that uncovered in previous studies, pointing to multiple mechanisms for achieving fixed transitions in flower color intensity. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/nph.14830 |
| الاتاحة: | http://dx.doi.org/10.1111/nph.14830 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fnph.14830 https://onlinelibrary.wiley.com/doi/pdf/10.1111/nph.14830 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.14830 https://nph.onlinelibrary.wiley.com/doi/am-pdf/10.1111/nph.14830 https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.14830 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#am ; http://onlinelibrary.wiley.com/termsAndConditions#vor ; http://doi.wiley.com/10.1002/tdm_license_1.1 ; http://onlinelibrary.wiley.com/termsAndConditions#am ; http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.1A5AEC78 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/nph.14830 |
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