Academic Journal
DIACYLGLYCEROL KINASE 5 participates in flagellin-induced signaling in Arabidopsis
| العنوان: | DIACYLGLYCEROL KINASE 5 participates in flagellin-induced signaling in Arabidopsis |
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| المؤلفون: | Kalachova, Tetiana, Škrabálková, Eliška, Pateyron, Stéphanie, Soubigou-Taconnat, Ludivine, Djafi, Nabila, Collin, Sylvie, Sekereš, Juraj, Burketová, Lenka, Potocký, Martin, Pejchar, Přemysl, Ruelland, Eric |
| المساهمون: | Institute of Experimental Botany of the Czech Academy of Sciences (IEB / CAS), Czech Academy of Sciences Prague (CAS), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Sorbonne Université (SU), GEC UTC (GEC UTC), Génie Enzymatique et Cellulaire (GEC), Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS)-Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS), European Regional Development Fund, Project “Centre for Experimental Plant Biology” grant no. CZ.02.1.01/0.0/0.0/16_019/0000738 the Czech Science Foundation GACR grant no. GA20-21547SMEYS CR (LM2018129 Czech-Bioimaging) and IEB. Czech-French cooperation was supported by the mobility program Barrande of the Czech Ministry of Education, Youth and Sports grant no. 8J20FR032 |
| المصدر: | ISSN: 0032-0889. |
| بيانات النشر: | CCSD American Society of Plant Biologists Oxford University Press |
| سنة النشر: | 2022 |
| المجموعة: | Université de Technologie de Compiègne: HAL |
| مصطلحات موضوعية: | Controlled natural selection, Plantmicrobiome interactions, Plant immunity, Pseudomonas syringae DC3000, Salicylic acid, Soil suppressiveness, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Abstract Flagellin perception is a keystone of pattern-triggered immunity in plants. The recognition of this protein by a plasma membrane (PM) receptor complex is the beginning of a signaling cascade that includes protein phosphorylation and the production of reactive oxygen species (ROS). In both Arabidopsis (Arabidopsis thaliana) seedlings and suspension cells, we found that treatment with flg22, a peptide corresponding to the most conserved domain of bacterial flagellin, caused a rapid and transient decrease in the level of phosphatidylinositol (PI) 4,5-bisphosphate along with a parallel increase in phosphatidic acid (PA). In suspension cells, inhibitors of either phosphoinositide-dependent phospholipases C (PLC) or diacylglycerol kinases (DGKs) inhibited flg22-triggered PA production and the oxidative burst. In response to flg22, receptor-like kinase-deficient fls2, bak1, and bik1 mutants (FLAGELLIN SENSITIVE 2, BRASSINOSTEROID INSENSITIVE 1-associated kinase 1, and BOTRYTIS-INDUCED KINASE 1, respectively) produced less PA than wild-type (WT) plants, whereas this response did not differ in NADPH oxidase-deficient rbohD (RESPIRATORY BURST OXIDASE HOMOLOG D) plants. Among the DGK-deficient lines tested, the dgk5.1 mutant produced less PA and less ROS after flg22 treatment compared with WT seedlings. In response to flg22, dgk5.1 plants showed lower callose accumulation and impaired resistance to Pseudomonas syringae pv. tomato DC3000 hrcC-. Transcriptomics revealed that the basal expression of defense-related genes was altered in dgk5.1 seedlings compared with the WT. A GFP-DGK5 fusion protein localized to the PM, where RBOHD and PLC2 (proteins involved in plant immunity) are also located. The role of DGK5 and its enzymatic activity in flagellin signaling and fine-tuning of early immune responses in plant–microbe interactions is discussed. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/35900211; PUBMED: 35900211; WOS: 000837792400001 |
| DOI: | 10.1093/plphys/kiac354 |
| الاتاحة: | https://hal.science/hal-03770162 https://hal.science/hal-03770162v1/document https://hal.science/hal-03770162v1/file/Kalachova%20et%20al.,%202022.pdf https://doi.org/10.1093/plphys/kiac354 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.19DB5874 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/plphys/kiac354 |
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