التفاصيل البيبلوغرافية
| العنوان: |
Surfactant-associated protein B kinetics in vivo in newborn infants by stable isotopes |
| المؤلفون: |
P. COGO, F. ROSSO, V. MORETTI, T. BADON, L. ZIMMERMANN, C. P. CARNIELLI, BARITUSSIO, ALDO, GUCCIARDI, ANTONINA, DUNER, ELENA |
| المساهمون: |
P., Cogo, Baritussio, Aldo, F., Rosso, Gucciardi, Antonina, V., Moretti, T., Badon, Duner, Elena, L., Zimmermann, C. P., Carnielli |
| سنة النشر: |
2005 |
| المجموعة: |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
| الوصف: |
Surfactant-associated protein B (SP-B) is critical to the biophysical function of pulmonary surfactant. No information is available on SP-B synthesis and kinetics in humans. We administered a 24-h i.v. infusion of 13C-valine as metabolic precursor of SP-B to six newborn infants (weight 3.5+/-0.5 kg; age 12 d, range 1-43 d). Three of the study infants also received i.v. 2H-palmitate to label surfactant disaturated phosphatidylcholine (DSPC). SP-B and DSPC were isolated from tracheal aspirates, and their respective 13C and 2H enrichments were measured by gas chromatography-mass spectrometry. SP-B kinetics was measured successfully in all six infants. SP-B median (range) fractional synthesis rate was 30% per day (20-78% per day), secretion time was 4.5 h (1-9 h), time to peak was 24 h (12-36 h), and half-life was 21 h (8-35 h). The ascending part of the SP-B kinetic curve was similar to the DSPC curve, suggesting similar secretion pathways. SP-B half-life seemed to be shorter than DSPC half-life. These results agree with existing animal data. We conclude that the measurement of SP-B kinetics is feasible in vivo in humans by stable isotope technology |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
info:eu-repo/semantics/altIdentifier/wos/WOS:000227746600009; volume:57; firstpage:519; lastpage:522; journal:PEDIATRIC RESEARCH; http://hdl.handle.net/11577/2463781 |
| الاتاحة: |
http://hdl.handle.net/11577/2463781 |
| رقم الانضمام: |
edsbas.19910648 |
| قاعدة البيانات: |
BASE |