Academic Journal
Assessing phage therapy against Pseudomonas aeruginosa using a Galleria mellonella infection model
| العنوان: | Assessing phage therapy against Pseudomonas aeruginosa using a Galleria mellonella infection model |
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| المؤلفون: | Beeton, Michael L., Alves, D. R., Enright, M. C., Jenkins, A. T. A. |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2015 |
| المجموعة: | Cardiff Metropolitan University: DSpace at Cardiff Met / Prifysgol Metropolitan Caerdydd |
| مصطلحات موضوعية: | pseudomonas aeruginosa, phage therapy, galleria mellonella, infection model |
| الوصف: | This article was published in International Journal of Antimicrobial Agents on 25th May 2015 (online), available at http://dx.doi.org/10.1016/j.ijantimicag.2015.04.005 ; The Galleria mellonella infection model was used to assess the in vivo efficacy of phage therapy against laboratory and clinical strains of Pseudomonas aeruginosa. In a first series of experiments, Galleria were infected with the laboratory strain P. aeruginosa PAO1 and were treated with varying multiplicity of infection (MOI) of phages either 2 h post-infection (treatment) or 2 h pre-infection (prevention) via injection into the haemolymph. To address the kinetics of infection, larvae were bled over a period of 24 h for quantification of bacteria and phages. Survival rates at 24 h when infected with 10 cells/larvae were greater in the prevention versus treatment model (47% vs. 40%, MOI = 10; 47% vs. 20%, MOI = 1; and 33% vs. 7%, MOI = 0.1). This pattern held true when 100 cells/larvae were used (87% vs. 20%, MOI = 10; 53% vs. 13%, MOI = 1; 67% vs. 7%, MOI = 0.1). By 24 h post-infection, phages kept bacterial cell numbers in the haemolymph 1000-fold lower than in the non-treated group. In a second series of experiments using clinical strains to further validate the prevention model, phages protected Galleria when infected with both a bacteraemia (0% vs. 85%) and a cystic fibrosis (80% vs. 100%) isolate. Therefore, this study validates the use of G. mellonella as a simple, robust and cost-effective model for initial in vivo examination of P. aeruginosa-targeted phage therapy, which may be applied to other pathogens with similarly low infective doses. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0924-8579 |
| Relation: | International Journal of Antimicrobial Agents; Beeton M. L., Alves. D. R., Enright M. C. and Jenkins A. T. A. (2016) ' Assessing phage therapy against Pseudomonas aeruginosa using a Galleria mellonella infection model', International Journal of Antimicrobial Agents, 46 (2), pp.196-200; http://hdl.handle.net/10369/7736; http://dx.doi.org/10.1016/j.ijantimicag.2015.04.005 |
| DOI: | 10.1016/j.ijantimicag.2015.04.005 |
| الاتاحة: | http://hdl.handle.net/10369/7736 https://doi.org/10.1016/j.ijantimicag.2015.04.005 |
| Rights: | Creative Commons Attribution Non-Commercial No Derivatives License ; https://creativecommons.org/licenses/by-nc-nd/3.0/ |
| رقم الانضمام: | edsbas.1910D7E |
| قاعدة البيانات: | BASE |
| تدمد: | 09248579 |
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| DOI: | 10.1016/j.ijantimicag.2015.04.005 |