Academic Journal
Emergence of Neuronal Diversity during Vertebrate Brain Development
| العنوان: | Emergence of Neuronal Diversity during Vertebrate Brain Development |
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| المؤلفون: | Raj, Bushra, Farrell, Jeffrey A., Liu, Jialin, El Kholtei, Jakob, Carte, Adam N., Navajas Acedo, Joaquin, Du, Lucia Y., McKenna, Aaron, Relić, Đorđe, Leslie, Jessica M., Schier, Alexander F. |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2020 |
| المجموعة: | University of Basel: edoc |
| الوصف: | Neurogenesis comprises many highly regulated processes including proliferation, differentiation, and maturation. However, the transcriptional landscapes underlying brain development are poorly characterized. We describe a developmental single-cell catalog of ∼220,000 zebrafish brain cells encompassing 12 stages from embryo to larva. We characterize known and novel gene markers for ∼800 clusters and provide an overview of the diversification of neurons and progenitors across these time points. We also introduce an optimized GESTALT lineage recorder that enables higher expression and recovery of Cas9-edited barcodes to query lineage segregation. Cell type characterization indicates that most embryonic neural progenitor states are transitory and transcriptionally distinct from neural progenitors of post-embryonic stages. Reconstruction of cell specification trajectories reveals that late-stage retinal neural progenitors transcriptionally overlap cell states observed in the embryo. The zebrafish brain development atlas provides a resource to define and manipulate specific subsets of neurons and to uncover the molecular mechanisms underlying vertebrate neurogenesis. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0896-6273 1097-4199 |
| Relation: | https://edoc.unibas.ch/79136/1/RajSchierBrain_Neuron_2020.pdf; Raj, Bushra and Farrell, Jeffrey A. and Liu, Jialin and El Kholtei, Jakob and Carte, Adam N. and Navajas Acedo, Joaquin and Du, Lucia Y. and McKenna, Aaron and Relić, Đorđe and Leslie, Jessica M. and Schier, Alexander F. (2020) Emergence of Neuronal Diversity during Vertebrate Brain Development. Neuron, 108 (6). pp. 1058-1074.e6.; info:pmid/33068532; info:isi/000603423200006; urn:ISSN:0896-6273; urn:ISSN:1097-4199 |
| DOI: | 10.1016/j.neuron.2020.09.023 |
| الاتاحة: | https://edoc.unibas.ch/79136/ https://edoc.unibas.ch/79136/1/RajSchierBrain_Neuron_2020.pdf https://doi.org/10.1016/j.neuron.2020.09.023 |
| Rights: | cc_by_nc_nd ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.18F5FA71 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 08966273 10974199 |
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| DOI: | 10.1016/j.neuron.2020.09.023 |