Academic Journal
Presentation of cartilage proteoglycan to a T cell hybridoma derived from a mouse with proteoglycan-induced arthritis
| العنوان: | Presentation of cartilage proteoglycan to a T cell hybridoma derived from a mouse with proteoglycan-induced arthritis |
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| المؤلفون: | BRENNAN, F R, NEGROIU, G, BUZÁS, E I, FÜLÖP, C, HOLLÓ, K, MIKECZ, K, GLANT, T T |
| المصدر: | Clinical and Experimental Immunology ; volume 100, issue 1, page 104-110 ; ISSN 1365-2249 0009-9104 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | Immunology, Immunology and Allergy |
| الوصف: | SUMMARY Immunization of BALB/c mice with human fetal cartilage proteoglycan (PG) produces progressive polyarthritis, and T ceils play key roles in the development of the disease. To gain an understanding of how PG is presented to autoreactive T cells by synovial antigen-presenting cells (APC), we examined the abilities of various syngeneic APC in presenting PG to a specific T ceil hybridoma 5/4E8, derived from a mouse with PG-induced arthritis. A20 B lymphoma cells and spleen cells were strong presenters of PG, but synoviocytes and P388D1 macrophages could only present PG effectively after stimulation with interferon-gamma (IFN-γ). The IFN-γ exerted its effect by up-regulating both MHC class II and intercellular adhesion molecule-1 (ICAM-1) expression by these cells as neutralizing antibodies to Ia, LFA-1 and ICAM-1 inhibited presentation. Our studies also showed that synoviocytes and spleen cells took up and processed PG more rapidly than the cell lines. Cysteine and serine protease-dependent antigen presentation of PG was blocked at 4°C, 18°C and by chloroquine treatment, indicating that presentation required active uptake and processing in an acidic compartment, probably in lysosomes. Also, keratan sulphate-depleted and cyanogen bromide (CNBr) and 2-nitro-5-thiocyanobenzoic acid (NTCB)-cleaved PG elicited stronger T cell responses, as they were more easily processed than the native molecule. Furthermore, CNBr-generated peptides were presented by fixed APC, indicating that core protein fragments of cartilage PG can be presented directly by APC in context with MHC class II molecules. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/j.1365-2249.1995.tb03610.x |
| الاتاحة: | http://dx.doi.org/10.1111/j.1365-2249.1995.tb03610.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2249.1995.tb03610.x https://academic.oup.com/cei/article-pdf/100/1/104/42202530/j.1365-2249.1995.tb03610.x.pdf |
| Rights: | https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
| رقم الانضمام: | edsbas.18B8C2E2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1365-2249.1995.tb03610.x |
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