التفاصيل البيبلوغرافية
| العنوان: |
Data_Sheet_1_Plant Species-Dependent Increased Abundance and Diversity of IncP-1 Plasmids in the Rhizosphere: New Insights Into Their Role and Ecology.pdf |
| المؤلفون: |
Masaki Shintani, Eman Nour, Tarek Elsayed, Khald Blau, Inessa Wall, Sven Jechalke, Cathrin Spröer, Boyke Bunk, Jörg Overmann, Kornelia Smalla |
| سنة النشر: |
2020 |
| المجموعة: |
Frontiers: Figshare |
| مصطلحات موضوعية: |
Microbiology, Microbial Genetics, Microbial Ecology, Mycology, lettuce-, tomato-, and potato rhizosphere, korB qPCR, IncP-1 plasmid, PacBio sequencing |
| الوصف: |
IncP-1 plasmids, first isolated from clinical specimens (R751, RP4), are recognized as important vectors spreading antibiotic resistance genes. The abundance of IncP-1 plasmids in the environment, previously reported, suggested a correlation with anthropogenic pollution. Unexpectedly, qPCR-based detection of IncP-1 plasmids revealed also an increased relative abundance of IncP-1 plasmids in total community DNA from the rhizosphere of lettuce and tomato plants grown in non-polluted soil along with plant age. Here we report the successful isolation of IncP-1 plasmids by exploiting their ability to mobilize plasmid pSM1890. IncP-1 plasmids were captured from the rhizosphere but not from bulk soil, and a high diversity was revealed by sequencing 14 different plasmids that were assigned to IncP-1β, δ, and ε subgroups. Although backbone genes were highly conserved and mobile elements or remnants as Tn501, IS1071, Tn402, or class 1 integron were carried by 13 of the sequenced IncP-1 plasmids, no antibiotic resistance genes were found. Instead, seven plasmids had a mer operon with Tn501-like transposon and five plasmids contained putative metabolic gene clusters linked to these mobile elements. In-depth sequence comparisons with previously known plasmids indicate that the IncP-1 plasmids captured from the rhizosphere are archetypes of those found in clinical isolates. Our findings that IncP-1 plasmids do not always carry accessory genes in unpolluted rhizospheres are important to understand the ecology and role of the IncP-1 plasmids in the natural environment. |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/dataset/Data_Sheet_1_Plant_Species-Dependent_Increased_Abundance_and_Diversity_of_IncP-1_Plasmids_in_the_Rhizosphere_New_Insights_Into_Their_Role_and_Ecology_pdf/13294241 |
| DOI: |
10.3389/fmicb.2020.590776.s001 |
| الاتاحة: |
https://doi.org/10.3389/fmicb.2020.590776.s001
https://figshare.com/articles/dataset/Data_Sheet_1_Plant_Species-Dependent_Increased_Abundance_and_Diversity_of_IncP-1_Plasmids_in_the_Rhizosphere_New_Insights_Into_Their_Role_and_Ecology_pdf/13294241 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.180772D8 |
| قاعدة البيانات: |
BASE |