Academic Journal
A fed-batch fermentation process for poly (3-hydroxybutyrate-co-3-hydroxyvalerate) production by Yangia sp. ND199 using molasses as substrate
| العنوان: | A fed-batch fermentation process for poly (3-hydroxybutyrate-co-3-hydroxyvalerate) production by Yangia sp. ND199 using molasses as substrate |
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| المؤلفون: | Thuoc, Doan Van, Phong, Tran Huu, Khuong, Dang Minh |
| المصدر: | TAP CHI SINH HOC; Vol 37, No 3 (2015); 325-331 ; Academia Journal of Biology; Vol 37, No 3 (2015); 325-331 ; 2615-9023 |
| بيانات النشر: | Publishing House for Science and Technology |
| سنة النشر: | 2015 |
| المجموعة: | Vietnam Academy of Science and Technology: Journals Online |
| مصطلحات موضوعية: | Yangia, fed-batch fermentation, molasses, P(3HB-co-3HV) |
| الوصف: | A locally isolated halophilic bacterium, Yangia sp. ND199 was able to use molasses as substrate for copolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] production. Cell dry weight (CDW) of 6.37 g/l, P(3HB-co-3HV) content of 43.1 wt% and P(3HB-co-3HV) concentration of 2.75 g/l were obtained by Yangia sp. after 60 h of cultivation in flask. In a batch cultivation mode in a fermentor, the CDW was increased to 9.1 g/l but P(3HB-co-3HV) content was decreased to 37 wt%. Fed-batch fermentation with two different nutrient feeding strategies was used. High CDW of 54.8 g/l was obtained after 54 h of cultivation but P(3HB-co-3HV) content was still low (39.8 wt%). Two-step fed-batch fermentation with two different nutrient feeding strategies was then designed. High CDW of 50 g/l and P(3HB-co-3HV) content of 52.9 wt% were obtained after 54 h of cultivation. The two-step fed-batch process designed here for the production of P(3HB-co-3HV) by Yangia sp. ND199 can be developed and used for further studies. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://vjs.ac.vn/index.php/vjbio/article/view/6465/6468; http://vjs.ac.vn/index.php/vjbio/article/view/6465 |
| DOI: | 10.15625/0866-7160/v37n3.6465 |
| الاتاحة: | http://vjs.ac.vn/index.php/vjbio/article/view/6465 https://doi.org/10.15625/0866-7160/v37n3.6465 |
| رقم الانضمام: | edsbas.17F8888A |
| قاعدة البيانات: | BASE |
| DOI: | 10.15625/0866-7160/v37n3.6465 |
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