التفاصيل البيبلوغرافية
| العنوان: |
Ciliary localization studies. |
| المؤلفون: |
Corvin D. Arveseth (10688311), John T. Happ (10688314), Danielle S. Hedeen (10688317), Ju-Fen Zhu (10688320), Jacob L. Capener (10688323), Dana Klatt Shaw (10688326), Ishan Deshpande (9943676), Jiahao Liang (8085302), Jiewei Xu (715341), Sara L. Stubben (10688329), Isaac B. Nelson (10688332), Madison F. Walker (10688335), Kouki Kawakami (10688338), Asuka Inoue (52011), Nevan J. Krogan (90155), David J. Grunwald (9997490), Ruth Hüttenhain (10688341), Aashish Manglik (1942735), Benjamin R. Myers (10688344) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Microbiology, Cell Biology, Genetics, Molecular Biology, Developmental Biology, Infectious Diseases, Biological Sciences not elsewhere classified, cascade drives, Smoothened transduces Hedgehog signals, organ development, SMO activates GLI, control expression, mechanism, subunit, SMO intracellular domains, activates glioma-associated, GLI transcription, membrane, activity-dependent sequestration, GPCR kinase, transcription factors, GRK, Hh signal transduction, pathway target genes, PKA-mediated inhibition, sequestering protein kinase |
| الوصف: |
Related to Fig 7 . Smo −/− fibroblasts expressing tdTomato-tagged ( A ) SMO-Nb fusions, or ( B ) the SMO Ala mutant were visualized via microscopy, following staining with antibodies to detect cilia (Arl13b), indicated in green. SMO is indicated in magenta. Representative images are shown, and the intensities of the ciliary SMO signals for wild-type SMO vs. the Ala mutant were quantified in ( C ) as described in “Methods” ( n = 30 cilia per condition). DAPI counterstain (blue) marks the nucleus. Scale bar = 2.5 μm. The underlying data for this figure can be found under S7 Data . Nb, nanobody; SMO, Smoothened. (PDF) |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Ciliary_localization_studies_/14470519 |
| DOI: |
10.1371/journal.pbio.3001191.s011 |
| الاتاحة: |
https://doi.org/10.1371/journal.pbio.3001191.s011 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.17E2735D |
| قاعدة البيانات: |
BASE |