Academic Journal
Fermentation of Vaccinium floribundum Berries with Lactiplantibacillus plantarum Reduces Oxidative Stress in Endothelial Cells and Modulates Macrophages Function
| العنوان: | Fermentation of Vaccinium floribundum Berries with Lactiplantibacillus plantarum Reduces Oxidative Stress in Endothelial Cells and Modulates Macrophages Function |
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| المؤلفون: | Marracino, Luisa, Punzo, Angela, Severi, Paolo, Nganwouo Tchoutang, Rosane, Vargas-De-la-Cruz, Celia, Fortini, Francesca, Vieceli Dalla Sega, Francesco, Silla, Alessia, Porru, Emanuele, Simoni, Patrizia, Rosta, Valentina, Trentini, Alessandro, Ouambo Talla, Achille Wilfred, Hrelia, Silvana, Cervellati, Carlo, Rizzo, Paola, Caliceti, Cristiana |
| المساهمون: | Marracino, Luisa, Punzo, Angela, Severi, Paolo, Nganwouo Tchoutang, Rosane, Vargas-De-la-Cruz, Celia, Fortini, Francesca, Vieceli Dalla Sega, Francesco, Silla, Alessia, Porru, Emanuele, Simoni, Patrizia, Rosta, Valentina, Trentini, Alessandro, Ouambo Talla, Achille Wilfred, Hrelia, Silvana, Cervellati, Carlo, Rizzo, Paola, Caliceti, Cristiana |
| سنة النشر: | 2022 |
| المجموعة: | Università degli Studi di Ferrara: CINECA IRIS |
| مصطلحات موضوعية: | lactic acid bacteria (LAB), fermentation, Pushgay (Vaccinium floribundum) berries, antioxidant activity, endothelial dysfunction, immunostimulant activity |
| الوصف: | Accumulating evidence suggests that high consumption of natural antioxidants promotes health by reducing oxidative stress and, thus, the risk of developing cardiovascular diseases. Similarly, fermentation of natural compounds with lactic acid bacteria (LAB), such as Lactiplantibacillus plantarum, enhances their beneficial properties as regulators of the immune, digestive, and cardiovascular system. We investigated the effects of fermentation with Lactiplantibacillus plantarum on the antioxidant and immunomodulatory effects of Pushgay berries (Vaccinium floribundum, Ericaceae family) in human umbilical vein endothelial cells (HUVECs) and macrophage cell line RAW264.7. Polyphenol content was assayed by Folin-Ciocalteu and HPLC-MS/MS analysis. The effects of berries solutions on cell viability or proliferation were assessed by WST8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt and Lactate dehydrogenase (LDH) release, Trypan blue exclusion test, and Alamar blue assay. Antioxidant activity was evaluated by a cell-based chemiluminescent probe for the detection of intracellular H2O2 production in HUVECs. Heme oxygenase-1 (HO-1) expression levels were investigated by RT-qPCR. Glutathione reductase (GR), glutathione peroxidase (Gpx), superoxide dismutase (SOD), and catalase (CAT) activities, as markers of intracellular antioxidant defense, were evaluated by spectrophotometric analysis. The immunomodulatory activity was examined in RAW 264.7 by quantification of inducible nitric oxide synthase (iNOS) and Tumor Necrosis Factor-alpha (TNF alpha) by RT-qPCR. Data showed that fermentation of Pushgay berries (i) enhances the content of quercetin aglycone, and (ii) increases their intracellular antioxidant activity, as indicated by the reduction in H2O2-induced cell death and the decrease in H2O2-induced HO-1 gene expression in HUVECs treated for 24 h with fermented berries solution (10 mu g/mL). Moreover, treatment with Pushgay berries for 72 h (10 mu g/mL) promotes cells ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/35458122; info:eu-repo/semantics/altIdentifier/wos/WOS:000785361700001; volume:14; issue:8; firstpage:1560-1; lastpage:1560-19; numberofpages:19; journal:NUTRIENTS; https://hdl.handle.net/11392/2503106; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85127908829; https://www.mdpi.com/2072-6643/14/8/1560 |
| DOI: | 10.3390/nu14081560 |
| الاتاحة: | https://hdl.handle.net/11392/2503106 https://doi.org/10.3390/nu14081560 https://www.mdpi.com/2072-6643/14/8/1560 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.17D4E470 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/nu14081560 |
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