التفاصيل البيبلوغرافية
| العنوان: |
Design and Production of Customizable and Highly Aligned Fibrillar Collagen Scaffolds |
| المؤلفون: |
Cassandra L. Martin (11420181), Chenxi Zhai (5345816), Jeffrey A. Paten (2024089), Jingjie Yeo (1515577), Leila F. Deravi (2592115) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Biotechnology, Evolutionary Biology, Immunology, Space Science, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, targeting experimental variables, simultaneously aligns type, mechanistic insights gleaned, fibrous collagenous materials, mild shear flow, shear rate, vitro <, taken together, studies inspired, spontaneously polymerize, shelf instrumentation, readily adapted, natural propensity, monomeric collagen, initial polymerization, help understand, easily amenable, chain density, also employed |
| الوصف: |
The ability to fabricate anisotropic collagenous materials rapidly and reproducibly has remained elusive despite decades of research. Balancing the natural propensity of monomeric collagen (COL) to spontaneously polymerize in vitro with the mild processing conditions needed to maintain its native substructure upon polymerization introduces challenges that are not easily amenable with off-the-shelf instrumentation. To overcome these challenges, we have designed a platform that simultaneously aligns type I COL fibrils under mild shear flow and builds up the material through layer-by-layer assembly. We explored the mechanisms propagating fibril alignment, targeting experimental variables such as shear rate, viscosity, and time. Coarse-grained molecular dynamics simulations were also employed to help understand how initial reaction conditions including chain length, indicative of initial polymerization, and chain density, indicative of concentration, in the reaction environment impact fibril growth and alignment. When taken together, the mechanistic insights gleaned from these studies inspired the design, iteration, fabrication, and then customization of the fibrous collagenous materials, illustrating a platform material that can be readily adapted to future tissue engineering applications. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Design_and_Production_of_Customizable_and_Highly_Aligned_Fibrillar_Collagen_Scaffolds/16606434 |
| DOI: |
10.1021/acsbiomaterials.1c00566.s001 |
| الاتاحة: |
https://doi.org/10.1021/acsbiomaterials.1c00566.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.17BB34BB |
| قاعدة البيانات: |
BASE |