Academic Journal
JHE in Gryllus assimilis: Cloning, sequence-activity associations and phylogeny
| العنوان: | JHE in Gryllus assimilis: Cloning, sequence-activity associations and phylogeny |
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| المؤلفون: | Crone, E. J., Zera, A. J., Anand, A., Oakeshott, J. G., Sutherland, T. D., Russell, R. J., Harshman, L. G., Hoffmann, F. G., Claudianos, C. |
| المساهمون: | A.S. Raikhel, R. Feyereisen |
| بيانات النشر: | Pergamon-Elsevier Science |
| سنة النشر: | 2007 |
| المجموعة: | The University of Queensland: UQ eSpace |
| مصطلحات موضوعية: | Biochemistry & Molecular Biology, Entomology, Juvenile hormone esterase gene, Gryllus assimilis, Hemimetabolous insect, Indel polymorphism, Phylogeny, 270205 Genetic Development (incl. Sex Determination), C1, 730210 Environmental health |
| الوصف: | The 458 amino acid sequence of a mature JHE protein from the cricket Gryllus assimilis was identified after isolating the partial cDNA sequence encoding this protein from a fat body and midgut cDNA library. This hemimetabolan JHE sequence shows over 40% amino acid similarity to the known JHE sequences of several holometabolous insects. It also includes previously determined peptide sequences for G. assimilis JHE as well as two other motifs associated with JHE enzymes in holometabolous insects. The predicted molecular weight of the protein agrees with that of the JHE previously purified from G. assimilis. Partial genomic sequence encoding the Jhe contains two large (1330 and 2918 bp) introns. No coding DNA sequence variation was observed over a 1293 bp region between selected lines differing six to eight-fold in hemolymph JHE activity. However, a 19 bp indel was found in one of the introns; the insertion was strongly associated with elevated hemolymph activity, both in the selected lines and in the F progeny of crosses between them. Phylogenetic analyses localised the G. assimilis JHE to a clade containing dipteran and coleopteran JHEs, with lepidopteran JHEs occurring in a separate clade. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0965-1748 1879-0240 |
| الاتاحة: | https://espace.library.uq.edu.au/view/UQ:130790 |
| رقم الانضمام: | edsbas.17922BB9 |
| قاعدة البيانات: | BASE |
| تدمد: | 09651748 18790240 |
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