Academic Journal
Studies on European eel skin sulfated glycosaminoglycans: Recovery, structural characterization and anticoagulant activity
| العنوان: | Studies on European eel skin sulfated glycosaminoglycans: Recovery, structural characterization and anticoagulant activity |
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| المؤلفون: | Sila A, Bougatef H, Capitani F, Krichen F, Mantovani V, Amor IB, Galeotti F, Maccari F, Nedjar N, Volpi N, Bougatef A. |
| المساهمون: | Sila, A, Bougatef, H, Capitani, F, Krichen, F, Mantovani, V, Amor, Ib, Galeotti, F, Maccari, F, Nedjar, N, Volpi, N, Bougatef, A. |
| سنة النشر: | 2018 |
| المجموعة: | Archivio della ricerca dell'Università di Modena e Reggio Emilia (Unimore: IRIS) |
| مصطلحات موضوعية: | Anguilla anguilla, Anticoagulant, Eel skin, Glycosaminoglycan, Structure |
| الوصف: | The goal of the present work was thè extraction and structural characterization of novel sulfated glycosaminoglycans from European eel skin. The recovered glycosaminoglycans were physicochemically characterized and thè uronic acid and sulfate contents were 35.12±2.13% and 16.32±0.4%, respectìvely. Cellulose acetate electrophoresis for extracted glycosaminoglycans was also investigated. Molecular weightof these sulfated glycosaminoglycans was determined (-37 kDa) by the gradient PAGE. Glycosaminoglycans obtained from thè European eel were composed of non-sulfated, mono- and disulfated disaccharides. These sulfated glycosaminoglycans were evaluated for their in vitro anticoagulant activity using activated partial thromboplastin time, thrombin time and prothrombin time tests. The result showed that thè recovered glycosaminoglycans exhibited interestingly anticoagulant activity. These glycosaminoglycans did not show haemolytic activity towards human erythrocytes. Furthermore, these bioactive substances can be explored as a functional food with antithrombotic function or used as source of anticoagulant drugs. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/29704607; info:eu-repo/semantics/altIdentifier/wos/WOS:000438662500101; volume:115; firstpage:891; lastpage:899; numberofpages:9; journal:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES; http://hdl.handle.net/11380/1164774; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85046483656 |
| DOI: | 10.1016/j.ijbiomac.2018.04.125 |
| الاتاحة: | http://hdl.handle.net/11380/1164774 https://doi.org/10.1016/j.ijbiomac.2018.04.125 |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.17221611 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.ijbiomac.2018.04.125 |
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