Academic Journal
In marine Bacteroidetes the bulk of glycan degradation during algae blooms is mediated by few clades using a restricted set of genes
| العنوان: | In marine Bacteroidetes the bulk of glycan degradation during algae blooms is mediated by few clades using a restricted set of genes |
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| المؤلفون: | Krüger, Karen, Chafee, Meghan, Ben Francis, T, Glavina del Rio, Tijana, Becher, Dörte, Schweder, Thomas, Amann, Rudolf I, Teeling, Hanno |
| المساهمون: | Deutsche Forschungsgemeinschaft |
| المصدر: | The ISME Journal ; volume 13, issue 11, page 2800-2816 ; ISSN 1751-7362 1751-7370 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2019 |
| الوصف: | We investigated Bacteroidetes during spring algae blooms in the southern North Sea in 2010–2012 using a time series of 38 deeply sequenced metagenomes. Initial partitioning yielded 6455 bins, from which we extracted 3101 metagenome-assembled genomes (MAGs) including 1286 Bacteroidetes MAGs covering ~120 mostly uncultivated species. We identified 13 dominant, recurrent Bacteroidetes clades carrying a restricted set of conserved polysaccharide utilization loci (PULs) that likely mediate the bulk of bacteroidetal algal polysaccharide degradation. The majority of PULs were predicted to target the diatom storage polysaccharide laminarin, alpha-glucans, alpha-mannose-rich substrates, and sulfated xylans. Metaproteomics at 14 selected points in time revealed expression of SusC-like proteins from PULs targeting all of these substrates. Analyses of abundant key players and their PUL repertoires over time furthermore suggested that fewer and simpler polysaccharides dominated early bloom stages, and that more complex polysaccharides became available as blooms progressed. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41396-019-0476-y |
| الاتاحة: | http://dx.doi.org/10.1038/s41396-019-0476-y http://www.nature.com/articles/s41396-019-0476-y.pdf http://www.nature.com/articles/s41396-019-0476-y https://academic.oup.com/ismej/article-pdf/13/11/2800/55551300/41396_2019_article_476.pdf |
| Rights: | https://creativecommons.org/licenses/by/4.0/ ; https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.170CC709 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41396-019-0476-y |
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