Academic Journal
Vitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infection
| العنوان: | Vitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infection |
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| المؤلفون: | Castillo Ramírez, Jorge Andrés, Giraldo Giraldo, Diana Marcela, Rodenhuis Zybert, Izabela Agata, Smit, Jolanda, Urcuqui Inchima, Silvio |
| بيانات النشر: | Inmunovirología Oxford University Press Oxford, Estados Unidos |
| سنة النشر: | 2022 |
| المجموعة: | Universidad de Antioquia (UdeA): Biblioteca Digital |
| مصطلحات موضوعية: | Virus del Dengue, Dengue Virus, Macrófagos, Macrophages, Inmunidad Innata, Immunity, Innate, Vitamina D, Vitamin D, LL-37 |
| الوصف: | Epidemics of dengue, an acute and potentially severe disease caused by mosquito-borne dengue virus (DENV), pose a major challenge to clinicians and health care services across the sub(tropics). Severe disease onset is associated with a dysregulated inflammatory response to the virus, and there are currently no drugs to alleviate disease symptoms. LL-37 is a potent antimicrobial peptide with a wide range of immunoregulatory properties. In this study, we assessed the effect of LL-37 on DENV-2-induced responses in human monocyte-derived macrophages (MDMs). We show that simultaneous exposure of exogenous LL-37 and DENV-2 resulted in reduced replication of the virus in MDMs, while the addition of LL-37 postexposure to DENV-2 did not. Interestingly, the latter condition reduced the production of IL-6 and increased the expression of genes involved in virus sensing and antiviral response. Finally, we demonstrate that low endogenous levels and limited production of LL-37 in MDMs in response to DENV-2 infection can be increased by differentiating MDMs in the presence of Vitamin D (VitD3). Taken together, this study demonstrates that in addition to its antimicrobial properties, LL-37 has immunomodulatory properties in the curse of DENV infection and its production can be increased by VitD3. ; COL0012444 |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | Pathog. Dis.; https://hdl.handle.net/10495/29734 |
| DOI: | 10.1093/femspd/ftac014 |
| الاتاحة: | https://hdl.handle.net/10495/29734 https://doi.org/10.1093/femspd/ftac014 |
| Rights: | info:eu-repo/semantics/openAccess ; Atribución-NoComercial-CompartirIgual 2.5 Colombia ; http://creativecommons.org/licenses/by-nc-sa/2.5/co/ ; http://purl.org/coar/access_right/c_abf2 ; https://creativecommons.org/licenses/by-nc-sa/4.0/ |
| رقم الانضمام: | edsbas.16EA4D8A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/femspd/ftac014 |
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