التفاصيل البيبلوغرافية
| العنوان: |
IRF3 and NF-κB nuclear translocation during SARS-CoV-2 infection of Calu-3 lung epithelial cells. |
| المؤلفون: |
Christine Vazquez (6312764), Sydnie E. Swanson (11021719), Seble G. Negatu (11021722), Mark Dittmar (11021725), Jesse Miller (4377925), Holly R. Ramage (11021728), Sara Cherry (170695), Kellie A. Jurado (11021731) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Medicine, Microbiology, Cell Biology, Molecular Biology, Immunology, Cancer, Mental Health, Infectious Diseases, Virology, Biological Sciences not elsewhere classified, downregulates IFIT 1 protein expression, SARS-CoV -2, host mRNA translation, SARS-CoV -2 pathogenesis, mechanism, NSP 13, NSP 13 protein expression, TBK 1, NSP 1 antagonizes interferon, phospho-IRF 3 protein levels, SARS-CoV -2 bypasses, NSP 13 downregulates interferon, proteins NSP 1, syndrome coronavirus 2, interferon activation, NF, IRF 3 activation |
| الوصف: |
(A, C) Representative image of Calu-3 cells 30 hours post SARS-CoV-2 infection that were immunostained with anti-Spike SARS-CoV-2 (green), anti-IRF3 (A, red), or anti-NF-κB (C, red). Nuclei are counterstained with DAPI (blue) with nuclear translocation of IRF3 (A) or NF-κB (C) visualized through co-localization of DAPI signal with IRF3 (A) or NF-κB (C). White arrows point to neighboring cells that have nuclear translocation of IRF3 (A) or NF-κB (C). Light blue arrows point to infected cells that are activated for IRF3 transcription (A) or NF-κB (C). Scale bar: 50 μm. (B) Quantification of images from (A) with cells positive for SARS-CoV-2 that had nuclear translocation of IRF3. Data are displayed as mean ± SD (n = 5 images counted with at least 50 cells per image). Data were analyzed by one-way ANOVA; ****p < 0.0001 (D) . Quantification of images from (C) with cells positive for SARS-CoV-2 that had nuclear translocation of NF-κB. Data are displayed as mean ± SD (n = 5 images counted with at least 50 cells per image). Data were analyzed by one-way ANOVA; ****p < 0.0001. (TIF) |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/IRF3_and_NF-_B_nuclear_translocation_during_SARS-CoV-2_infection_of_Calu-3_lung_epithelial_cells_/14840968 |
| DOI: |
10.1371/journal.pone.0253089.s001 |
| الاتاحة: |
https://doi.org/10.1371/journal.pone.0253089.s001 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.16DC96C3 |
| قاعدة البيانات: |
BASE |