Academic Journal
Effect of Phosphatidylethanolamine on Pore Formation Induced by the Antimicrobial Peptide PGLa
| العنوان: | Effect of Phosphatidylethanolamine on Pore Formation Induced by the Antimicrobial Peptide PGLa |
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| المؤلفون: | Marzuk Ahmed, Md. Zahidul Islam, Md. Masum Billah, Masahito Yamazaki |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Biophysics, Biochemistry, Cell Biology, Molecular Biology, Pharmacology, Cancer, Computational Biology, Environmental Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, small vesicles appeared, giant unilamellar vesicles, causes severe leakage, nanopore formation started, induced nanopore formation, pore formation induced, induce pore formation, pgla forms nanopores, single guv method, lipid membrane aggregates, antimicrobial peptide pgla, induced local burst, dope )/ dopg, pore formation, local burst, lipid bilayers, guv membrane, antimicrobial peptides, time resolution, prepore rim |
| الوصف: | Most antimicrobial peptides (AMPs) induce pore formation and a burst of lipid bilayers and plasma membranes. This causes severe leakage of the internal contents and cell death. The AMP PGLa forms nanopores in giant unilamellar vesicles (GUVs) comprising dioleoylphosphatidylcholine (DOPC) and dioleoylphosphatidylglycerol (DOPG). We here elucidated the effect of the line tension of a prepore rim on PGLa-induced nanopore formation by investigating the interaction of PGLa with single GUVs comprising dioleoylphosphatidylethanolamine (DOPE)/DOPG (6:4) in buffer using the single GUV method. We found that PGLa forms nanopores in the GUV membrane, which evolved into a local burst and burst of GUVs. The rate of pore formation in DOPE/DOPG-GUVs was smaller than that in DOPC/DOPG-GUVs. PGLa is located only in the outer leaflet of a GUV bilayer just before a fluorescent probe AF647 leakage from the inside, indicating that this asymmetric distribution induces nanopore formation. PGLa-induced local burst and burst of GUVs were observed at 10 ms-time resolution. After nanopore formation started, dense particles and small vesicles appeared in the GUVs, followed by a decrease in the GUV diameter. The GUV was finally converted into smaller GUV or lipid membrane aggregates. We discuss the mechanisms of PGLa-induced nanopore formation and its direct evolution to a GUV burst. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/journal_contribution/Effect_of_Phosphatidylethanolamine_on_Pore_Formation_Induced_by_the_Antimicrobial_Peptide_PGLa/25358974 |
| DOI: | 10.1021/acs.jpcb.3c08098.s001 |
| الاتاحة: | https://doi.org/10.1021/acs.jpcb.3c08098.s001 https://figshare.com/articles/journal_contribution/Effect_of_Phosphatidylethanolamine_on_Pore_Formation_Induced_by_the_Antimicrobial_Peptide_PGLa/25358974 |
| Rights: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.16C03220 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.jpcb.3c08098.s001 |
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