Academic Journal
Effect of the CXCR4 antagonist plerixafor on endogenous neutrophil dynamics in the bone marrow, lung and spleen
| العنوان: | Effect of the CXCR4 antagonist plerixafor on endogenous neutrophil dynamics in the bone marrow, lung and spleen |
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| المؤلفون: | Pillay, J, Tregay, N, Juzenaite, G, Carlin, LM, Pirillo, C, Gaboriau, DCA, Farahi, N, Summers, C, Lo Celso, C, Chilvers, ER, Rankin, S, De Filippo, K |
| المساهمون: | Wellcome Trust |
| المصدر: | 1185 ; 1175 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2020 |
| المجموعة: | Imperial College London: Spiral |
| مصطلحات موضوعية: | Science & Technology, Life Sciences & Biomedicine, Cell Biology, Hematology, Immunology, neutrophil activation, neutrophil dynamics, neutrophil mobilization, CHEMOKINE RECEPTOR, RAPID MOBILIZATION, WHIM-SYNDROME, AMD3100, CELLS, RELEASE, MYELOKATHEXIS, RECRUITMENT, INHIBITION, PLATFORM, 0601 Biochemistry and Cell Biology, 1107 Immunology |
| الوصف: | Treatment with the CXCR4 antagonist, plerixafor (AMD3100), has been proposed for clinical use in patients with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome and in pulmonary fibrosis. However, there is controversy with respect to the impact of plerixafor on neutrophil dynamics in the lung, which may affect its safety profile. In this study, we investigated the kinetics of endogenous neutrophils by direct imaging, using confocal intravital microscopy in mouse bone marrow, spleen, and lungs. Neutrophils are observed increasing their velocity and exiting the bone marrow following plerixafor administration, with a concomitant increase in neutrophil numbers in the blood and spleen, while the marginated pool of neutrophils in the lung microvasculature remained unchanged in terms of numbers and cell velocity. Use of autologous radiolabeled neutrophils and SPECT/CT imaging in healthy volunteers showed that plerixafor did not affect GM‐CSF‐primed neutrophil entrapment or release in the lungs. Taken together, these data suggest that plerixafor causes neutrophil mobilization from the bone marrow but does not impact on lung marginated neutrophil dynamics and thus is unlikely to compromise respiratory host defense both in humans and mice. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 0741-5400 |
| Relation: | Journal of Leukocyte Biology; http://hdl.handle.net/10044/1/79588; 201356/Z/16/Z |
| DOI: | 10.1002/JLB.1MA0420-571RR |
| الاتاحة: | http://hdl.handle.net/10044/1/79588 https://doi.org/10.1002/JLB.1MA0420-571RR |
| Rights: | © 2020 The Authors. Journal of Leukocyte Biology published by Wiley Periodicals, Inc. on behalf of Society for Leukocyte Biology This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| رقم الانضمام: | edsbas.1671153D |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 07415400 |
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| DOI: | 10.1002/JLB.1MA0420-571RR |