Academic Journal
Characterization of the diversity of sulfate-reducing bacteria in soil and mining waste water environments by nucleic acid hybridization techniques
| العنوان: | Characterization of the diversity of sulfate-reducing bacteria in soil and mining waste water environments by nucleic acid hybridization techniques |
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| المؤلفون: | Telang, Anita J., Voordouw, Gerrit, Ebert, Sara, Sifeldeen, Neili, Foght, Julia M., Fedorak, Phillip M., Westlake, D. W. S. |
| المصدر: | Canadian Journal of Microbiology ; volume 40, issue 11, page 955-964 ; ISSN 0008-4166 1480-3275 |
| بيانات النشر: | Canadian Science Publishing |
| سنة النشر: | 1994 |
| الوصف: | Nucleic acid hybridization techniques were used to characterize the sulfate-reducing bacterial communities at seven waste water and two soil sites in Canada. Genomic DNA was obtained from liquid enrichment cultures of samples taken from these nine sites. The liquid enrichment protocol favored growth of the sulfate-reducing bacterial component of the communities at these sites. The genomic DNA preparations were analyzed with (i) a specific gene probe aimed at a single genus (Desulfovibrio), (ii) a general 16S rRNA gene probe aimed at all genera of sulfate-reducing bacteria and other bacteria, and (iii) whole genome probes aimed at specific bacteria. This three-pronged approach provided information on the sulfate-reducing bacterial community structures for the nine sites. These were compared with each other and with the sulfate-reducing bacterial communities of western Canadian oil field production waters, studied previously. It was found that there is considerable diversity in the sulfate-reducing bacterial community at each site. Most sulfate-reducing bacteria isolated from distinct sites are genomically different and differ also from sulfate-reducing bacteria found in oil field production waters.Key words: sulfate-reducing bacteria, genomic diversity, nucleic acid hybridization, microbial community. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1139/m94-152 |
| الاتاحة: | http://dx.doi.org/10.1139/m94-152 http://www.nrcresearchpress.com/doi/pdf/10.1139/m94-152 |
| Rights: | http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
| رقم الانضمام: | edsbas.166A1CBE |
| قاعدة البيانات: | BASE |
| DOI: | 10.1139/m94-152 |
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