Academic Journal
Exosomal miR-22-3p from Mesenchymal Stem Cells Inhibits the Epithelial-Mesenchymal Transition (EMT) of Melanoma Cells by Regulating LGALS1
| العنوان: | Exosomal miR-22-3p from Mesenchymal Stem Cells Inhibits the Epithelial-Mesenchymal Transition (EMT) of Melanoma Cells by Regulating LGALS1 |
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| المؤلفون: | Yong Chen, Yuan Fang, Li Li, Hui Luo, Tianran Cao, Biao Tu |
| المصدر: | Frontiers in Bioscience-Landmark, Vol 27, Iss 9, p 275 (2022) |
| بيانات النشر: | IMR Press |
| سنة النشر: | 2022 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | melanoma, epithelial-mesenchymal transition, lgals1, mir-22-3p, Biochemistry, QD415-436, Biology (General), QH301-705.5 |
| الوصف: | Background: The mortality rate from melanoma has been rising and hence new therapeutic approaches for this disease have received extensive attention, especially the search for novel therapeutic targets. The aim of this study was to find new targets for the treatment of melanoma through a bioinformatics and experimental approach. Methods: First, we screened for differentially expressed genes (DEGs) and differentially expressed miRNAs (DEMs) between melanoma and normal tissues using the TCGA-SKCM, GTEX, and GSE24996 datasets. Next, we identified epithelial-mesenchymal transition (EMT)-related DEGs and analyzed their expression levels and association with patient survival. The expression level of DEGs was then confirmed in normal human melanocytes and melanoma cells. Bioinformatics analysis was used to identify miRNAs that targeted the most highly expressed DEG, LGALS1, and their binding confirmed using dual luciferase. Enriched pathways for the LGALS1 target miR-22-3p were also analyzed. miR-22-3p was overexpressed in cells in order to investigate changes in cell activity and in related genes and proteins. Exosomes from human bone marrow mesenchymal stem cells (MSCs) were coated with miR-22-3p to examine its effect on EMT. Results: The expression levels of LGALS1, CPXM1, and APLNR were higher in melanoma than in normal tissues and were associated with worse patient survival. The differential expression of these genes was confirmed using normal human skin melanocytes (PIG1) and human melanoma cells (WM-266-4). LGALS1 was the most differentially expressed gene between WM-266-4 and PIG1 cells, and was also predicted to be a target for miR-22-3p. The results of dual luciferase experiments confirmed that miR-22-3p could bind to LGALS1. Following the overexpression of miR-22-3p in WM-266-4 cells, the cell viability decreased, the expression levels of LGALS1, VIM and SNAI2 decreased, the expression level of CDH1 increased, and cell apoptosis increased. Transfection of miR-22-3p using exosomes resulted in similar effects. ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2768-6701 |
| Relation: | https://www.imrpress.com/journal/FBL/27/9/10.31083/j.fbl2709275; https://doaj.org/toc/2768-6701; https://doaj.org/article/572b9ffe2d21441688edb3205986068a |
| DOI: | 10.31083/j.fbl2709275 |
| الاتاحة: | https://doi.org/10.31083/j.fbl2709275 https://doaj.org/article/572b9ffe2d21441688edb3205986068a |
| رقم الانضمام: | edsbas.161C12C7 |
| قاعدة البيانات: | BASE |
| تدمد: | 27686701 |
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| DOI: | 10.31083/j.fbl2709275 |