Academic Journal
Transferability of heterologous microsatellite primers in Leiarius marmoratus ; Transferibilidade de primers microssatélites heterólogos em Leiarius marmoratus
| العنوان: | Transferability of heterologous microsatellite primers in Leiarius marmoratus ; Transferibilidade de primers microssatélites heterólogos em Leiarius marmoratus |
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| المؤلفون: | Pandolfi, Victor César Freitas, Yamachita, Andrei Lincoln, Souza, Felipe Pinheiro de, Lima, Ed Christian Suzuki de, Ayres, Denise Rocha, Bignardi, Annaiza Braga, Povh, Jayme Aparecido, Pereira, Ulisses de Pádua, Urrea-Rojas, Angela Maria, Lopera-Barrero, Nelson Mauricio |
| المصدر: | Semina: Ciências Agrárias; Vol. 41 No. 5supl1 (2020); 2297-2306 ; Semina: Ciências Agrárias; v. 41 n. 5supl1 (2020); 2297-2306 ; 1679-0359 ; 1676-546X |
| بيانات النشر: | UEL |
| سنة النشر: | 2020 |
| المجموعة: | Universidade Estadual de Londrina: Portal de Periódicos Científicos da UEL |
| مصطلحات موضوعية: | Aquaculture, Amazonian Jundiá, Cross-amplification, Genetic conservation, SSR, Aquicultura, Amplificação cruzada, Conservação genética, Jundiá amazônico |
| الوصف: | The Amazonian Jundiá (Leiarius marmoratus) (Siluliformes: Pimelodidae) is a species of catfish with social and economic importance in some South American countries such as Brazil and Colombia. Genetic evaluation of this species is limited due to the lack of specific molecular markers, hindering studies on genetic diversity and structure in animals under captive conditions or in natural populations. The objective of the present study was to evaluate the transferability of heterologous microsatellite markers in Leiarius marmoratus. Thirty-two heterologous primers were tested in L. marmoratus. The primers that presented the best standards were applied to 20 specimens, and the number of alleles (Na), number of effective alleles (Ne), gene diversity per Locus (GdL) and percentage of amplification failure (Md) were calculated. Eleven primers demonstrated satisfactory transferability patterns, all from the fish of the Pimelodidae family, of which, seven were monomorphic and four polymorphic. The eleven markers presented low percentage of Md (mean was 5.9% samples per locus). Na varied from one to two alleles per locus, revealing low polymorphism in the evaluated samples. The mean Ne and GdL numbers were 1.77 and 0.32, respectively. The transferability of the heterologous microsatellite loci in L. marmoratus was shown to be possible. However, further tests are needed to apply these markers in population genetic studies. ; O Jundiá da Amazônia (Leiarius marmoratus) (Siluliformes: Pimelodidae) é uma espécie de catfish com importância social e econômica em alguns países Sul americanos como Brasil e Colômbia. A avaliação genética dessa espécie é limitada devido a ausência de marcadores moleculares específicos para L. marmoratus, dificultando estudos sobre a diversidade e estrutura genética em animais em condições de cativeiro ou em populações naturais. O objetivo do presente estudo foi avaliar a transferibilidade de 32 marcadores microssatélites heterólogos em Leiarius marmoratus. Os primers que apresentaram melhores ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38246/27806; https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38246 |
| DOI: | 10.5433/1679-0359.2020v41n5supl1p2297 |
| الاتاحة: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38246 https://doi.org/10.5433/1679-0359.2020v41n5supl1p2297 |
| Rights: | Copyright (c) 2020 Semina: Ciências Agrárias ; http://creativecommons.org/licenses/by-nc/4.0 |
| رقم الانضمام: | edsbas.1581B47E |
| قاعدة البيانات: | BASE |
| DOI: | 10.5433/1679-0359.2020v41n5supl1p2297 |
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