Academic Journal
Histochemical and biometric study of the gastrointestinal system of Hyla orientalis (Bedriaga, 1890) (Anura, Hylidae)
| العنوان: | Histochemical and biometric study of the gastrointestinal system of Hyla orientalis (Bedriaga, 1890) (Anura, Hylidae) |
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| المؤلفون: | Arikan, H., Akat, E., Gocmen, B. |
| بيانات النشر: | Pagepress Publ |
| سنة النشر: | 2014 |
| المجموعة: | Ege University Institutional Repository |
| مصطلحات موضوعية: | goblet cell, Gastrointestinal system, glycoproteins, hyaluronic acid, amphibian, Hyla |
| الوصف: | PubMed ID: 25578977 ; WOS: 000347228000005 ; This study was carried out to assess the localization of hyaluronic acid (HA) and the distribution of glycoproteins in the gastrointestinal system of adult Hyla orientalis. Histochemical analysis of the gastrointestinal system in H. orientalis showed that mucous content included glycogene and/or oxidable dioles [periodic acid/Schiff (PAS)+], neutral or acid-rich (PAS/AB pH 2.5+), sialic acid residues (KOH/PAS+) and acid sulphate [Aldehyde fuchsin (AF)+] glycoproteins. However the mucus content was not the same in stomach, small and large intestine. The mucus content of stomach included only glycogene and/or oxidable dioles and sialic acid residues. Besides these histochemical methods, the localization of HA was detected using biotinylated hyaluronic acid binding protein labeled with streptavidin-fluorescein isothiocyanate (FITC). In the extracellular matrix of the submucosa, the reaction for HA was evident. Since HA was located in submucosa beneath the epithelial layer of gastrointestinal system, it has a significant role in hydric balance, and essential to provide the gastrointestinal system integrity and functionality. According to biometric results, there were statistical differences between small and large intestine in terms of the amount of material stained positive with PAS/AB, PAS, KOH/PAS and AF/AB. Additionally, number of goblet cells in the small and large intestine was significantly different. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2038-8306 1121-760X |
| Relation: | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; European Journal of Histochemistry; https://hdl.handle.net/11454/48490; https://doi.org/10.4081/ejh.2014.2452; 58; 291; 295 |
| DOI: | 10.4081/ejh.2014.2452 |
| الاتاحة: | https://hdl.handle.net/11454/48490 https://doi.org/10.4081/ejh.2014.2452 |
| Rights: | open |
| رقم الانضمام: | edsbas.1562C23 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20388306 1121760X |
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| DOI: | 10.4081/ejh.2014.2452 |