Academic Journal
Synthesis of an oligonucleotide-derivatized amphipol and its use to trap and immobilize membrane proteins
| العنوان: | Synthesis of an oligonucleotide-derivatized amphipol and its use to trap and immobilize membrane proteins |
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| المؤلفون: | Bon, Christel Le, Della Pia, Eduardo Antonio, Giusti, Fabrice, Lloret, Noemie Denise Carmen, Zoonens, Manuela, Martinez, Karen Laurence, Popot, Jean Luc |
| المصدر: | Bon , C L , Della Pia , E A , Giusti , F , Lloret , N D C , Zoonens , M , Martinez , K L & Popot , J L 2014 , ' Synthesis of an oligonucleotide-derivatized amphipol and its use to trap and immobilize membrane proteins ' , Nucleic Acids Research , vol. 42 , no. 10 , e83 . https://doi.org/10.1093/nar/gku250 |
| سنة النشر: | 2014 |
| المجموعة: | University of Copenhagen: Research / Forskning ved Københavns Universitet |
| الوصف: | Amphipols (APols) are specially designed amphipathic polymers that stabilize membrane proteins (MPs) in aqueous solutions in the absence of detergent. A8-35, a polyacrylate-based APol, has been grafted with an oligodeoxynucleotide (ODN). The synthesis, purification and properties of the resulting 'OligAPol' have been investigated. Grafting was performed by reacting an ODN carrying an amine-terminated arm with the carboxylates of A8-35. The use of OligAPol for trapping MPs and immobilizing them onto solid supports was tested using bacteriorhodopsin (BR) and the transmembrane domain of Escherichia coli outer membrane protein A (tOmpA) as model proteins. BR and OligAPol form water-soluble complexes in which BR remains in its native conformation. Hybridization of the ODN arm with a complementary ODN was not hindered by the assembly of OligAPol into particles, nor by its association with BR. BR/OligAPol and tOmpA/OligAPol complexes could be immobilized onto either magnetic beads or gold nanoparticles grafted with the complementary ODN, as shown by spectroscopic measurements, fluorescence microscopy and the binding of anti-BR and anti-tOmpA antibodies. OligAPols provide a novel, highly versatile approach to tagging MPs, without modifying them chemically nor genetically, for specific, reversible and targetable immobilization, e.g. for nanoscale applications. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.1093/nar/gku250 |
| الاتاحة: | https://curis.ku.dk/portal/da/publications/synthesis-of-an-oligonucleotidederivatized-amphipol-and-its-use-to-trap-and-immobilize-membrane-proteins(97ab74c2-7aef-4894-9dce-dd05e9e5cea1).html https://doi.org/10.1093/nar/gku250 https://curis.ku.dk/ws/files/132424799/Nucl._Acids_Res._2014_Le_Bon_e83.pdf |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.154B72FB |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/nar/gku250 |
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