Academic Journal
Gemcitabine induces poly (ADP-Ribose) polymerase-1 (PARP-1) degradation through autophagy in pancreatic cancer
| العنوان: | Gemcitabine induces poly (ADP-Ribose) polymerase-1 (PARP-1) degradation through autophagy in pancreatic cancer |
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| المؤلفون: | Wang, Yufeng, Kuramitsu, Yasuhiro, Tokuda, Kazuhiro, Baron, Byron, Kitagawa, Takao, Akada, Junko, Maehara, Shin-ichiro, Maehara, Yoshihiko, Nakamura, Kazuyuki |
| بيانات النشر: | Public Library of Science |
| سنة النشر: | 2014 |
| المجموعة: | University of Malta: OAR@UM / L-Università ta' Malta |
| مصطلحات موضوعية: | DNA repair, DNA damage, Pancreas -- Cancer, Protein kinases, Cell death, Cancer cells -- Effect of drugs on, Medical biochemistry |
| الوصف: | Poly (ADP-ribose) polymerase-1 (PARP-1) and autophagy play increasingly important roles in DNA damage repair and cell death. Gemcitabine (GEM) remains the first-line chemotherapeutic drug for pancreatic cancer (PC). However, little is known about the relationship between PARP-1 expression and autophagy in response to GEM. Here we demonstrate that GEM induces DNA-damage response and degradation of mono-ADP ribosylated PARP-1 through the autophagy pathway in PC cells, which is rescued by inhibiting autophagy. Hypoxia and serum starvation inhibit autophagic activity due to abrogated GEM-induced mono-ADP-ribosylated PARP-1 degradation. Activation of extracellular regulated protein kinases (ERK) induced by serum starvation shows differences in intracellular localization as well as modulation of autophagy and PARP-1 degradation in GEM-sensitive KLM1 and -resistant KLM1-R cells. Our study has revealed a novel role of autophagy in PARP-1 degradation in response to GEM, and the different impacts of MEK/ERK signaling pathway on autophagy between GEMsensitive and -resistant PC cells. ; peer-reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | Wang, Y., Kuramitsu, Y., Tokuda, K., Baron, B., Kitagawa, T., Akada, J., . & Nakamura, K. (2014). Gemcitabine induces poly (ADP-ribose) polymerase-1 (PARP-1) degradation through autophagy in pancreatic cancer. PLoS One, 9(10), e109076.; https://www.um.edu.mt/library/oar/handle/123456789/98629 |
| DOI: | 10.1371/journal.pone.0109076 |
| الاتاحة: | https://www.um.edu.mt/library/oar/handle/123456789/98629 https://doi.org/10.1371/journal.pone.0109076 |
| Rights: | info:eu-repo/semantics/openAccess ; The copyright of this work belongs to the author(s)/publisher. The rights of this work are as defined by the appropriate Copyright Legislation or as modified by any successive legislation. Users may access this work and can make use of the information contained in accordance with the Copyright Legislation provided that the author must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the prior permission of the copyright holder. |
| رقم الانضمام: | edsbas.1530274F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0109076 |
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