Academic Journal

Molecular Signatures of Immune Activation and Epithelial Barrier Remodeling Are Enhanced during the Luteal Phase of the Menstrual Cycle: Implications for HIV Susceptibility

التفاصيل البيبلوغرافية
العنوان: Molecular Signatures of Immune Activation and Epithelial Barrier Remodeling Are Enhanced during the Luteal Phase of the Menstrual Cycle: Implications for HIV Susceptibility
المؤلفون: Birse, K., Arnold, KB, Novak, RM, McCorrister, S., Shaw, S., Westmacott, GR, Ball, TB, Lauffenburger, DA, Burgener, A.
بيانات النشر: American Society for Microbiology
سنة النشر: 2015
المجموعة: University of Illinois at Chicago: UIC INDIGO (INtellectual property in DIGital form available online in an Open environment)
الوصف: This is the copy of an article published in the Journal of Virology © 2015 American Society for Microbiology Publications. ; The variable infectivity and transmissibility of HIV/SHIV has been recently associated with the menstrual cycle, with particular susceptibility observed during the luteal phase in nonhuman primate models and ex vivo human explant cultures, but the mechanism is poorly understood. Here, we performed an unbiased, mass spectrometry-based proteomic analysis to better understand the mucosal immunological processes underpinning this observed susceptibility to HIV infection. Cervicovaginal lavage samples (n 19) were collected, characterized as follicular or luteal phase using days since last menstrual period, and analyzed by tandem mass spectrometry. Biological insights from these data were gained using a spectrum of computational methods, including hierarchical clustering, pathway analysis, gene set enrichment analysis, and partial least-squares discriminant analysis with LASSO feature selection. Of the 384 proteins identified, 43 were differentially abundant between phases (P < 0.05, >2-fold change). Cell-cell adhesion proteins and antiproteases were reduced, and leukocyte recruitment (interleukin-8 pathway, P 1.41E–5) and extravasation proteins (P 5.62E–4) were elevated during the luteal phase. LASSO/PLSDA identified a minimal profile of 18 proteins that best distinguished the luteal phase. This profile included cytoskeletal elements and proteases known to be involved in cellular movement. Gene set enrichment analysis associated CD4 T cell and neutrophil gene set signatures with the luteal phase (P < 0.05). Taken together, our findings indicate a strong association between proteins involved in tissue remodeling and leukocyte infiltration with the luteal phase, which may represent potential hormone-associated mechanisms of increased susceptibility to HIV. IM
نوع الوثيقة: article in journal/newspaper
اللغة: unknown
تدمد: 0022-538X
Relation: Birse, K., Arnold, K. B., Novak, R. M., McCorrister, S., Shaw, S., Westmacott, G. R., Ball, T. B., Lauffenburger, D. A. and Burgener, A. Molecular signatures of immune activation and epithelial barrier remodeling are enhanced during the luteal phase of the menstrual cycle: Implications for HIV susceptibility. Journal of Virology. 2015. 89(17): 8793-8805. DOI:10.1128/JVI.00756-15.; http://hdl.handle.net/10027/20015
الاتاحة: http://hdl.handle.net/10027/20015
رقم الانضمام: edsbas.14EF1C32
قاعدة البيانات: BASE