التفاصيل البيبلوغرافية
| العنوان: |
Phylogenetic analyses of the hematophagous bat Desmodus rotundus in Valle del Cauca, Colombia ; Análisis filogenético del murciélago hematófago Desmodus rotundus en el Valle del Cauca Colombia |
| المؤلفون: |
Castro castro, Fernando Favian, Muñoz Flores, Jaime Eduardo, Uieda, Wilson |
| المصدر: |
Acta Agronómica; Vol. 65 No. 1 (2016); 65 - 71 ; Acta Agronómica; Vol. 65 Núm. 1 (2016); 65 - 71 ; Acta Agronómica; v. 65 n. 1 (2016); 65 - 71 ; 2323-0118 ; 0120-2812 |
| بيانات النشر: |
Universidad Nacional de Colombia - Sede Palmira |
| سنة النشر: |
2016 |
| المجموعة: |
Universidad Nacional de Colombia: Portal de Revistas UN |
| مصطلحات موضوعية: |
DNA, clades, Genbank, Haplotypes, patagium, PCR, 16SL, 16SH, Animal science, animal biotechnology, ecology, natural sciences, earth sciences, ADN, Clados Genbank, Haplotipos, Patagio, Ciencia animal, genética animal, biología molecular, ciencias naturales |
| الوصف: |
Phylogenetic analyses of the hematophagous bat Desmodus rotundus were performed in villages belonging to Valle del Cauca, Cauca and Amazonas regions. Samples were mainly collected at hematophagous bat shelters located in Victoria, Águila, Obando, Cartago, Zarzal, San Pedro, Cerrito and Palmira villages belonging to Valle del Cauca region, two samples at Mercaredes which belong to Cauca, two samples at Puerto Nariño which is part of Amazonas region. Epithelial tissue samples were collected from bat’s patagium, and submitted to PCR using primers 16 SL and 16SH amplifying mitochondrial DNA of 50 vampires, these products were sequencing resulting in eight haplotypes being the most common presented in 43 individuals. Two methods to reach maximum likelihood and parsimony were used. GENBANK sequences of mitochondrial markers s16 rRNA from Brazil, Venezuela and Costa Rica were used for comparison. Higher similarity between vampire bats from Valle del Cauca and Cauca region, located in the southwest Colombia and vampire bats from Costa Rica and Venezuela was found. In the present study the genetic profiles correlated with the phylogeographic aspects of different populations was performed and a connection between different haplotypes of existing clades was found according to the phylogenetic tree and haplotype net, possible being generated during hematophagous bats migration over the mountain-chain and rivers crossing Colombia. ; En los municipios La Victoria, Águila, Obando, Cartago, Zarzal, San Pedro, Cerrito y Palmira del depar-tamento Valle del Cauca; Mercaderes en el Cauca; y Puerto Nariño en el departamento del Amazonas Amazonas, se realizó el análisis de filogenia del murciélago hematófago Desmodus rotundus. Las mues-tras de tejido epitelial fueron tomadas en el patagio del quiróptero, fueron amplificadas por PCR utilizan-do cebadores 16SL y 16SH, se amplificó ADN mitocondrial, y se secuenciaron 50 individuos de murciéla-gos hematófagos. Después de la secuenciación se encontraron ocho haplotipos y se encontró que ... |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf; text/html |
| اللغة: |
Spanish; Castilian |
| Relation: |
https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/47496/52252; https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/47496/55247; https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/47496 |
| الاتاحة: |
https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/47496 |
| Rights: |
Derechos de autor 2016 Acta Agronómica ; https://creativecommons.org/licenses/by-nc-nd/4.0 |
| رقم الانضمام: |
edsbas.14B3E21C |
| قاعدة البيانات: |
BASE |