التفاصيل البيبلوغرافية
| العنوان: |
Comparison between coagulation factor VIII quantified with one-stage activity assay and with mass spectrometry in haemophilia A patients: Proof of principle |
| المؤلفون: |
Donners, Anouk A.M.T., van Maarseveen, Erik M., Weetink, Yrea R.J., El Amrani, Mohsin, Fischer, Kathelijn, Rademaker, Carin M.A., Egberts, Toine C.G., Huisman, Albert, Musson, Ruben E.A. |
| المساهمون: |
Apotheek Opleiding, Apotheek Klinische Farmacie, Apotheek KF TDM, Poli Van Creveldkliniek Medisch, Child Health, Circulatory Health, Brain, Infection & Immunity, Regenerative Medicine and Stem Cells, Apotheek Onderzoek, CDL Poli WKZ, CDL Staf Patiëntenzorg KC, Other research (not in main researchprogram) |
| سنة النشر: |
2020 |
| مصطلحات موضوعية: |
blood coagulation tests, factor VIII, haemophilia A, mass spectrometry, proof of concept, Hematology, Clinical Biochemistry, Biochemistry, medical, Journal Article |
| الوصف: |
Introduction: Haemophilia A is a hereditary bleeding disorder caused by a factor VIII (FVIII) deficiency. As biomarker, FVIII activity is used to classify disease severity and to monitor treatment. The one-stage clotting assay (OSA) is performed to measure FVIII activity, but OSA's limitations may result in misclassification of disease severity or suboptimal monitoring of treatment. Measurement of FVIII plasma concentration with liquid chromatography-tandem mass spectrometry (LC-MS/MS) might overcome these challenges. The objective is to investigate the correlation between FVIII activity and concentration, and determinants for differences between the two methods. Methods: In this cross-sectional study, all haemophilia A patients receiving standard-of-care were eligible for inclusion. Within the activity categories of <1 IU/dL, 1-5 IU/dL, >5-40 IU/dL, >40-150 IU/dL and >150-600 IU/dL, we randomly selected 15-20 plasma samples and compared FVIII concentration (LC-MS/MS) to FVIII activity (OSA) with linear regression and Bland-Altman analysis. Potential determinants for differences were analysed with linear regression. Results: Inclusion was 87 samples. Bland-Altman analysis demonstrated an overall mean difference of −1% with an SD of 64% between the two methods. Large differences were correlated with the presence of anti-FVIII antibodies (133% [95% CI: 81, 185] n = 5) and use of exogenous FVIII products (−37% [95% CI: −65,-9] n = 58), for example plasma-derived and B-domain–modified FVIII products. Conclusions: Despite good overall correlation between the two methods, relative differences were large, especially for samples with anti-FVIII antibodies or exogenous FVIII products. These differences may have clinical impact. More research is needed to determine the value of FVIII plasma concentration in comparison with FVIII activity. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| تدمد: |
1751-5521 |
| Relation: |
https://dspace.library.uu.nl/handle/1874/441019 |
| الاتاحة: |
https://dspace.library.uu.nl/handle/1874/441019 |
| Rights: |
info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: |
edsbas.14B22422 |
| قاعدة البيانات: |
BASE |