Academic Journal
Interdependence of Shigella flexneri O Antigen and Enterobacterial Common Antigen Biosynthetic Pathways
| العنوان: | Interdependence of Shigella flexneri O Antigen and Enterobacterial Common Antigen Biosynthetic Pathways |
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| المؤلفون: | Maczuga, N., Tran, E.N.H., Qin, J., Morona, R. |
| المساهمون: | El-Naggar, M.Y. |
| المصدر: | http://dx.doi.org/10.1128/jb.00546-21. |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2022 |
| المجموعة: | The University of Adelaide: Digital Library |
| مصطلحات موضوعية: | Shigella flexneri, Lipopolysaccharides, O Antigens, Antigens, Bacterial, Biosynthetic Pathways |
| الوصف: | Outer membrane (OM) polysaccharides allow bacteria to resist harsh environmental conditions and antimicrobial agents, traffic to and persist in pathogenic niches, and evade immune responses. Shigella flexneri has two OM polysaccharide populations, being enterobacterial common antigen (ECA) and lipopolysaccharide (LPS) O antigen (Oag); both are polymerized into chains by separate homologs of the Wzy-dependent pathway. The two polysaccharide pathways, along with peptidoglycan (PG) biosynthesis, compete for the universal biosynthetic membrane anchor, undecaprenyl phosphate (Und-P), as the finite pool of available Und-P is critical in all three cell wall biosynthetic pathways. Interactions between the two OM polysaccharide pathways have been proposed in the past where, through the use of mutants in both pathways, various perturbations have been observed. Here, we show for the first time that mutations in one of the two OM polysaccharide pathways can affect each other, dependent on where the mutation lies along the pathway, while the second pathway remains genetically intact. We then expand on this and show that the mutations also affect PG biosynthesis pathways and provide data which supports that the classical mutant phenotypes of cell wall mutants are due to a lack of available Und-P. Our work here provides another layer in understanding the complex intricacies of the cell wall biosynthetic pathways and demonstrates their interdependence on Und-P, the universal biosynthetic membrane anchor. ; Nicholas Maczuga, Elizabeth N. H. Tran, Jilong Qin, Renato Morona |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0021-9193 1098-5530 |
| Relation: | http://purl.org/au-research/grants/arc/DP160103903; Journal of Bacteriology, 2022; 204(4):1-16; https://hdl.handle.net/2440/134866; Maczuga, N. [0000-0001-7638-2613]; Tran, E.N.H. [0000-0003-1644-2287]; Morona, R. [0000-0001-7009-7440] |
| DOI: | 10.1128/jb.00546-21 |
| الاتاحة: | https://hdl.handle.net/2440/134866 https://doi.org/10.1128/jb.00546-21 |
| Rights: | © 2022 American Society for Microbiology. All Rights Reserved. |
| رقم الانضمام: | edsbas.14A1A8EC |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00219193 10985530 |
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| DOI: | 10.1128/jb.00546-21 |