التفاصيل البيبلوغرافية
| العنوان: |
Cooperativity in binding processes: new insights from phenomenological modeling |
| المؤلفون: |
Cattoni, Diego Ignacio, Chara, Osvaldo, Kaufman, Sergio Benjamín, Gonzalez Flecha, Francisco Luis |
| بيانات النشر: |
Public Library Of Science |
| المجموعة: |
CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
| مصطلحات موضوعية: |
ligand binding, kinetic models, thermodynamics, protein interactions, https://purl.org/becyt/ford/1.6, https://purl.org/becyt/ford/1 |
| الوصف: |
Cooperative binding is one of the most interesting and not fully understood phenomena involved in control and regulation of biological processes. Here we analyze the simplest phenomenological model that can account for cooperativity (i.e. ligand binding to a macromolecule with two binding sites) by generating equilibrium binding isotherms from deterministically simulated binding time courses. We show that the Hill coefficients determined for cooperative binding, provide a good measure of the Gibbs free energy of interaction among binding sites, and that their values are independent of the free energy of association for empty sites. We also conclude that although negative cooperativity and different classes of binding sites cannot be distinguished at equilibrium, they can be kinetically differentiated. This feature highlights the usefulness of pre-equilibrium time-resolved strategies to explore binding models as a key complement of equilibrium experiments. Furthermore, our analysis shows that under conditions of strong negative cooperativity, the existence of some binding sites can be overlooked, and experiments at very high ligand concentrations can be a valuable tool to unmask such sites. ; Fil: Cattoni, Diego Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universite Montpellier II; Francia ; Fil: Chara, Osvaldo. Universite Montpellier II; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina ; Fil: Kaufman, Sergio Benjamín. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de ... |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| تدمد: |
1932-6203 |
| Relation: |
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0146043; http://hdl.handle.net/11336/18338; Cattoni, Diego Ignacio; Chara, Osvaldo; Kaufman, Sergio Benjamín; Gonzalez Flecha, Francisco Luis; Cooperativity in binding processes: new insights from phenomenological modeling; Public Library Of Science; Plos One; 10; 12; 12-2015; 1-14; e0146043; CONICET Digital; CONICET |
| الاتاحة: |
http://hdl.handle.net/11336/18338 |
| Rights: |
info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
| رقم الانضمام: |
edsbas.148896DB |
| قاعدة البيانات: |
BASE |