Academic Journal
A DNA Element Regulates Drug Tolerance and Withdrawal in Drosophila
| العنوان: | A DNA Element Regulates Drug Tolerance and Withdrawal in Drosophila |
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| المؤلفون: | Li, Xiaolei, Ghezzi, Alfredo, Pohl, Jascha B., Bohm, Arun Y., Atkinson, Nigel S. |
| بيانات النشر: | PLOS One |
| سنة النشر: | 2013 |
| المجموعة: | The University of Texas at Austin: Texas ScholarWorks |
| مصطلحات موضوعية: | alcohols, calcium-activated potassium channels, epileptic seizures, mutation, phenotype, polymerase chain reaction, sedatives |
| الوصف: | Drug tolerance and withdrawal are insidious responses to drugs of abuse; the first increases drug consumption while the second punishes abstention. Drosophila generate functional tolerance to benzyl alcohol sedation by increasing neural expression of the slo BK-type Ca2+ activated K+ channel gene. After drug clearance this change produces a withdrawal phenotype—increased seizure susceptibility. The drug-induced histone modification profile identified the 6b element (60 nt) as a drug responsive element. Genomic deletion of 6b produces the allele, sloΔ6b, that reacts more strongly to the drug with increased induction, a massive increase in the duration of tolerance, and an increase in the withdrawal phenotype yet does not alter other slo-dependent behaviors. The 6b element is a homeostatic regulator of BK channel gene expression and is the first cis-acting DNA element shown to specifically affect the duration of a drug action. ; This work has been supported by National Institutes of Health (NIH) R01 AA018037 (subgroup: NIAAA, title: Epigenetic Modification as a Mechanism to Produce Functional Tolerance). URL: http://www.niaaa.nih.gov. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; Waggoner Center for Alcohol and Addiction Research ; Email: NigelA@mail.utexas.edu |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | Li X, Ghezzi A, Pohl JB, Bohm AY, Atkinson NS (2013) A DNA Element Regulates Drug Tolerance and Withdrawal in Drosophila. PLoS ONE 8(9): e75549. doi:10.1371/journal.pone.0075549; http://hdl.handle.net/2152/27780; journal.pone.0075549.pdf |
| DOI: | 10.1371/journal.pone.0075549 |
| الاتاحة: | http://hdl.handle.net/2152/27780 https://doi.org/10.1371/journal.pone.0075549 |
| Rights: | Administrative deposit of works to UT Digital Repository: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access at http://www.plosone.org. The public license is specified as CC-BY: http://creativecommons.org/licenses/by/4.0/. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University. |
| رقم الانضمام: | edsbas.147ED7E2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0075549 |
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