التفاصيل البيبلوغرافية
| العنوان: |
Validation of the RPGRIP1L antibody. |
| المؤلفون: |
Poppy Datta (159540), J. Thomas Cribbs (10765801), Seongjin Seo (198289) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Medicine, Cell Biology, Genetics, Molecular Biology, Neuroscience, Immunology, Developmental Biology, Hematology, Biological Sciences not elsewhere classified, IMPG, Nphp 1 gene-trap, ablation causes nephronophthisis, SNAP, NPHP 1 patients, compartmentalized protein localization, NPHP 1, ciliary transition zone protein, segment development, segment plasma membrane proteins, interphotoreceptor matrix proteogly., photoreceptor maturation period, Cep 290 gene dose results, STX |
| الوصف: |
hTERT-RPE1 cells (ATCC #CRL-4000) were transfected with control (siCTRL; Horizon ON-TARGETplus Non-targeting Pool cat#: D-001810-10-05) or human RPGRIP1L siRNAs (siRPGRIP1L; Horizon ON-TARGETplus SMARTpool cat# L-022557-00-0005) using Lipofectamine RNAiMAX (Invitrogen), and cell lysates were loaded on an SDS-PAGE gel for immunoblotting with anti-RPGRIP1L antibodies. β-actin was used as a loading control. RPGRIP1L band was marked with a red arrowhead. (TIF) |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Validation_of_the_RPGRIP1L_antibody_/14557174 |
| DOI: |
10.1371/journal.pone.0246358.s002 |
| الاتاحة: |
https://doi.org/10.1371/journal.pone.0246358.s002 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.144BB8C6 |
| قاعدة البيانات: |
BASE |