Academic Journal

Liquid chromatographic determination of polyphenenols in Czech beers during brewing proces

التفاصيل البيبلوغرافية
العنوان: Liquid chromatographic determination of polyphenenols in Czech beers during brewing proces
المؤلفون: Ganbaatar, Chunsriimyatav, Kubáň, Vlastimil, Kráčmar, Stanislav, Valášek, Pavel, Fišera, Miroslav, Hoza, Ignác
المصدر: Potravinárstvo
بيانات النشر: HACCP Consulting
سنة النشر: 2015
المجموعة: Tomas Bata University Zlin: TBU publications / Univerzita Tomáše Bati ve Zlíně
مصطلحات موضوعية: Beers, Brewing technology, HPLC, Polyphenols, UV-VIS diode array detection, Worts
الوصف: High performance liquid chromatographic (HPLC/UV) method was adapted for simultaneous determination of seven polyphenols, including derivatives of benzoic (gallic and vanillic acids) and cinnamic acids (p-coumaric, ferulic and sinapic acids), flavan-3-ols (catechin) and flavonols (rutin) in worts and beers at the various stages of the brewing process. Based on the semi-quantitative HPLC analysis, total polyphenols chromatographic index (TPCI) was in the ranges of 5.18 - 19.4 mg/L and 7.37 - 20.7 mg/L for all worts and beers, respectively. The HPLC analyses showed that relatively high levels of (+)-catechin and gallic acid were in all the worts and the beers, while the values were much lower for ferulic acid, rutin, vanillic acid, sinapic acid and p-coumaric acid. Polyphenols with relatively high concentrations, that were detected in all tested worts and beers, were gallic acid (1.29 - 4.75 mg/L resp. 2.59 - 4.97 mg/L), (+)-catechin (1.66 - 7.95 mg/L resp. 4.70 - 10.0 mg/L) and ferulic acid (0.41 - 4.53 mg/L resp. 1.05 - 2.87 mg/L). On the other side, the sinapic acid (0.72 - 1.59 mg/L resp. 0.72 - 2.5 mg/L), rutin (1.17 - 2.03 mg/L resp. 1.16 - 2.85 mg/L), p-coumaric acid (ND - 4.73 mg/L resp. ND - 1.44 mg/L) and vanillic acid (ND - 1.52 mg/L resp. 0.75 - 1.81 mg/L) were detected in lowest concentrations. In both, worts and beers investigated in this study, the changes in the contents of individual polyphenols were not uniform. In the case of some polyphenols, a decrease in the content was observed after boiling the worts with hops or after the main fermentation until maturation and filtration, but with some polyphenols, the concentrations were constant until the end of the process or even increased. © 2015 Potravinarstvo. All rights reserved.
نوع الوثيقة: article in journal/newspaper
اللغة: English
Relation: http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/viewArticle/421; Potravinárstvo; http://publikace.k.utb.cz/handle/10563/1006300
الاتاحة: http://publikace.k.utb.cz/handle/10563/1006300
Rights: Attribution 3.0 International ; https://creativecommons.org/licenses/by/3.0/ ; openAccess
رقم الانضمام: edsbas.140D6540
قاعدة البيانات: BASE