Academic Journal
AXENIC CULTIVATION OF THE HETEROTROPHIC DINOFLAGELLATE PFIESTERIA SHUMWAYAE AND OBSERVATIONS ON FEEDING BEHAVIOR 1
| العنوان: | AXENIC CULTIVATION OF THE HETEROTROPHIC DINOFLAGELLATE PFIESTERIA SHUMWAYAE AND OBSERVATIONS ON FEEDING BEHAVIOR 1 |
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| المؤلفون: | Skelton, Hayley M., Burkholder, JoAnn M., Parrow, Matthew W. |
| المصدر: | Journal of Phycology ; volume 44, issue 6, page 1614-1624 ; ISSN 0022-3646 1529-8817 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2008 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Pfiesteria shumwayae Glasgow et J. M. Burkh. [= Pseudopfiesteria shumwayae (Glasgow et J. M. Burkh.) Litaker, Steid., P. L. Mason, Shields et P. A. Tester] is a heterotrophic dinoflagellate commonly found in temperate, estuarine waters. P. shumwayae can feed on other protists, fish, and invertebrates, but research on the biochemical requirements of this species has been restricted by the lack of axenic cultures. An undefined, biphasic culture medium was formulated that supported the axenic growth of two of three strains of P. shumwayae . The medium contained chicken egg yolk as a major component. Successful growth depended on the method used to sterilize the medium, and maximum cell yields (10 4 · mL −1 ) were similar to those attained in previous research when P. shumwayae was cultured with living fish or microalgae. Additionally, P. shumwayae flagellate cells ingested particles present in the biphasic medium, allowing detailed observations of feeding behavior. This research is an initial step toward a chemically defined axenic culture medium and determination of P. shumwayae metabolic requirements. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/j.1529-8817.2008.00601.x |
| الاتاحة: | http://dx.doi.org/10.1111/j.1529-8817.2008.00601.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1529-8817.2008.00601.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1529-8817.2008.00601.x |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.1390911B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1529-8817.2008.00601.x |
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