Academic Journal
Essential role of C/EBPα in G‐CSF‐induced transcriptional activation and chromatin modification of myeloid‐specific genes
| العنوان: | Essential role of C/EBPα in G‐CSF‐induced transcriptional activation and chromatin modification of myeloid‐specific genes |
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| المؤلفون: | Iida, Satoshi, Watanabe‐Fukunaga, Rie, Nagata, Shigekazu, Fukunaga, Rikiro |
| المصدر: | Genes to Cells ; volume 13, issue 4, page 313-327 ; ISSN 1356-9597 1365-2443 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2008 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Granulocyte colony‐stimulating factor (G‐CSF) regulates the proliferation and differentiation of neutrophilic progenitor cells. Here, we investigated the roles of CCAAT/enhancer‐binding protein (C/EBP)α in the G‐CSF‐induced transcriptional activation and chromatin modification of the CCR2 and myeloperoxidase (MPO) genes in IL‐3‐dependent myeloid FDN1.1 cells. Chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assays revealed that G‐CSF activates C/EBPα to bind target promoters. ChIP mapping experiments across the CCR2 and MPO genes showed that G‐CSF induces histone H3 modifications: the acetylation of Lys9, trimethylation of Lys4 and trimethylation of Lys9. The distribution profile of the trimethylated Lys9 was distinct from that of the two other modifications. All the G‐CSF‐induced C/EBPα recruitment, transcriptional activation and histone modifications were reversed by re‐stimulation with IL‐3, and were abolished by short hairpin RNA (shRNA)‐mediated knockdown of C/EBPα. These results indicate that C/EBPα is activated by G‐CSF to bind target promoters, and plays critical roles in the transcriptional activation and dynamic chromatin modification of target genes during neutrophil differentiation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/j.1365-2443.2008.01173.x |
| الاتاحة: | https://doi.org/10.1111/j.1365-2443.2008.01173.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2443.2008.01173.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2443.2008.01173.x |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.1383B180 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1365-2443.2008.01173.x |
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