التفاصيل البيبلوغرافية
| العنوان: |
Identifying Mechanisms Used by Adherent-invasive Escherichia coli Associated with Crohn Disease to Evade the Immune System |
| المؤلفون: |
Ossa, Juan C. |
| المساهمون: |
Sherman, Philip M., Medical Science |
| بيانات النشر: |
University of Toronto |
| المجموعة: |
University of Toronto: Research Repository T-Space |
| مصطلحات موضوعية: |
Crohn Disease (CD), Adherent-Invasive Escherichia coli (AIEC), Interferon gamma (IFNγ), Signal transducer and activator of transcription 1 (STAT1) |
| Time: |
0410, 0307, 0982 |
| الوصف: |
Background: Adherent-invasive Escherichia coli (AIEC) is a pathogen isolated from the ileum of patients with CD. IFNγ is a key mediator of immunity, which regulates inflammatory responses to microbial infections. Previously, we showed enterohemorrhagic E. coli prevents STAT1 activation. Aims: To determine; 1) whether activation of STAT1 by IFNγ was prevented following AIEC infection, and 2) define the mechanisms used. Methods: Human epithelial cells were infected with AIEC strains or other pathogenic and commensal E. coli strains. Following infection, cells were stimulated with IFNγ. Activation of STAT1, was monitored by immunoblotting. Results: AIEC strains prevented STAT1 phosphorylation in response to IFNγ. Effect required live bacteria with active protein synthesis. A bacterial product was responsible for blocking STAT1 signalling and interfered with downstream signalling cascades. Conclusion: Suppression of epithelial cell STAT1 signal transduction by AIEC strains represents a novel mechanism by which the pathogen evades host immune responses to the infection. ; MAST |
| نوع الوثيقة: |
thesis |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
http://hdl.handle.net/1807/32615 |
| الاتاحة: |
http://hdl.handle.net/1807/32615 |
| رقم الانضمام: |
edsbas.133BA1EE |
| قاعدة البيانات: |
BASE |