Conference
In vivo selection of multidrug resistance in Enterobacter cloacae complex by a unique romR deletion
| العنوان: | In vivo selection of multidrug resistance in Enterobacter cloacae complex by a unique romR deletion |
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| المؤلفون: | Gravey, François, Ethuin, F, Cattoir, V., Beyrouthy, R., Bonnet, R., Le Hello, S., Guérin, Fabien |
| المساهمون: | Groupe de Recherche sur l'Adaptation Microbienne (GRAM 2.0), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Service d'Anesthésie - Réanimation Chirurgicale CHU Caen, Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), Centre Hospitalier Universitaire de Rennes CHU Rennes = Rennes University Hospital Ponchaillou, CHU Clermont-Ferrand, Hôpital Côte de Nacre CHU Caen, CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN), Service de Microbiologie CHU Caen, RIN Normandie, European Society of Clinical Microbiology and Infectious Diseases |
| المصدر: | 29th European Congress of Clinical Microbiology & Infectious Diseases https://normandie-univ.hal.science/hal-02406906 29th European Congress of Clinical Microbiology & Infectious Diseases, Apr 2019, Amsterdam, Netherlands. , 2019 https://2019.eccmid.org/ |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2019 |
| المجموعة: | Normandie Université: HAL |
| مصطلحات موضوعية: | Enterobacter cloacae, Enterobacter cloacae complex, Bioinformatics, Antibiotic resistance, RomR, Carbapenem, Cephalosporin, Temocillin, Tigecyclin, Chloramphenicol, Long-term antibiotic treatment, MESH: Enterobacter cloacae, MESH: Biologie informatique, MESH: Résistance microbienne aux médicaments, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases |
| جغرافية الموضوع: | Amsterdam, Netherlands |
| الوصف: | International audience ; Objectives: Members of the Enterobacter cloacae complex (ECC) are major opportunistic pathogens, especially among intensive care unit (ICU) patients. The aim of this study was to decipher the phenotypic and genomic evolution of an ECC clone that acquired a multidrug resistance phenotype in an ICU patient. Methods: During a four-month period, 8 MDR ECC strains were recovered from the same patient: 5 from clinical samples and 3 from rectal swabs. The patient benefited from an escalation of antibiotic treatment until he died. MIC values of 18 antibiotics were determined by the broth microdilution method. Whole-genome sequencing was performed for the 8 strains and a Single-Nucleotide Polymorphism (SNP) analysis was performed after alignment against the ECC reference genome (ATCC 13047). Results: In strains isolated from the 3rd month, a significant increase in MICs (at least 4 fold change) was observed for ceftazidime, cefepime, temocillin, ertapenem, tigecycline and chloramphenicol. All strains harbored the same antibiotic resistance genes against β-lactams, aminoglycosides, fluoroquinolones, sulphonamides, trimethoprime and chloramphenicol. The 8 MDR ECC belonged to the same cluster 6 (ECC6) and were genetically related. No genetic evolution was found in chromosomal genes usually involved in β-lactam resistance (i.e. ampD, ampR, nagZ, dacA, dacB, acrAB-tolC, ompK35, ompK36). The isolates differed by 5 SNPs located in metabolism genes and a deletion of 13 bases in romR, which encodes for TetR-type transcriptonal regulator. This latter is known to play a repressor role on the expression of porins such as ompK35, whose the downregulation might be involved in MIC increases observed here. Conclusion: Consequent and long-term antibiotic treatment in one patient indicated the selection of a unique MDR ECC6 population where antibiotic resistance evolved more by chromosomal modifications than acquired antibiotic resistance genes. The analysis of the different genetic features through all the 8 MDR ... |
| نوع الوثيقة: | conference object still image |
| اللغة: | English |
| Relation: | hal-02406906; https://normandie-univ.hal.science/hal-02406906; https://normandie-univ.hal.science/hal-02406906/document; https://normandie-univ.hal.science/hal-02406906/file/ESCMID_RomR_poster.pdf |
| الاتاحة: | https://normandie-univ.hal.science/hal-02406906 https://normandie-univ.hal.science/hal-02406906/document https://normandie-univ.hal.science/hal-02406906/file/ESCMID_RomR_poster.pdf |
| Rights: | http://creativecommons.org/licenses/by-nc/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.13223203 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |