Academic Journal
Differential expression of prostaglandin (PG) synthesis enzymes in conceptus during peri-implantation period and endometrial expression of carbonyl reductase/PG 9-ketoreductase in the pig
| العنوان: | Differential expression of prostaglandin (PG) synthesis enzymes in conceptus during peri-implantation period and endometrial expression of carbonyl reductase/PG 9-ketoreductase in the pig |
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| المؤلفون: | Waclawik, Agnieszka, Ziecik, Adam J |
| المصدر: | Journal of Endocrinology ; volume 194, issue 3, page 499-510 ; ISSN 0022-0795 1479-6805 |
| بيانات النشر: | Bioscientifica |
| سنة النشر: | 2007 |
| الوصف: | Prostaglandins (PGs) play a pivotal role in luteolysis, maternal recognition of pregnancy, and implantation. In many species, including pigs, both conceptus (embryo and associated membranes) and endometrium synthesize PGE 2 , which may antagonize PGF 2α by playing a luteotropic/antiluteolytic role. Previously, we have reported expression profiles of PG G/H synthases (PGHS-1 and PGHS-2), PGE synthase (mPGES-1), and PGF synthase (PGFS) in the endometrium of cyclic and pregnant pigs. In the present study, expression of above-mentioned PG synthesis enzymes and PG 9-ketoreductase (CBR1), which converts PGE 2 into PGF 2α , and the PGE 2 /PGF 2α ratios were investigated in porcine peri- and post-implantation conceptuses. Furthermore, expression of CBR1 was examined in the endometrium. PGHS-2 and mPGES-1 were upregulated, and PGHS-1, PGFS, and CBR1 were downregulated in conceptuses during trophoblastic elongation. A second increase of mPGES-1 mRNA occurred after days 20–21 of pregnancy. After initiation of implantation, expression of PGHS-1, PGFS, and CBR1 in conceptuses increased and remained higher until days 24–25 of pregnancy. Comparison of the endometrial CBR1 protein expression in cyclic and pregnant gilts revealed upregulation on days 16–17 of the cycle and downregulation on days 10–11 of pregnancy. In conclusion, reciprocal expression of PGHS-2, mPGES-1, PGFS, and CBR1 in day 10–13 conceptuses and decrease of endometrial CBR1 may be important in increasing the PGE 2 /PGF 2α ratio during maternal recognition of pregnancy. This study indicates that PGE 2 produced via PGHS-2 and mPGES-1 in conceptus may be involved in corpus luteum control. Moreover, high expression of conceptus PGHS-1, mPGES-1, PGFS, and CBR1 after initiation of implantation suggests their significant role in placentation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| DOI: | 10.1677/joe-07-0155 |
| الاتاحة: | http://dx.doi.org/10.1677/joe-07-0155 https://joe.bioscientifica.com/view/journals/joe/194/3/1940499.xml https://joe.bioscientifica.com/downloadpdf/journals/joe/194/3/1940499.xml |
| رقم الانضمام: | edsbas.131FE041 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1677/joe-07-0155 |
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