Academic Journal
SUMMARY
| العنوان: | SUMMARY |
|---|---|
| المؤلفون: | Cell Surface, W. E. G. Mcller, R. K. Zahn, B. Kurelec |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://jcs.biologists.org/content/21/2/227.full.pdf. |
| المجموعة: | CiteSeerX |
| الوصف: | An aggregation receptor from the siliceous sponge Geodia cydonium has been isolated and purified in an almost pure form. It sediments at about 2-6 s, has a buoyant density of 151 g/ml in CsCl and elutes from Sephadex G-50 at a V,jV0 value of i- 3 i i. Chemical analysis revealed that the receptor consists of 81 % neutral carbohydrate and 7-5 % protein. The activity of the receptor is rapidly destroyed by Na-periodate. The receptor is released from the cell surface after removal of Ca2+ from the medium or after incubation of the cells with trypsin. The depleted cells can be charged again with isolated receptor molecules. The binding of the receptor molecules on the cell surface is prevented in the presence of trypsin. For optimal binding, physiological salt concentrations with respect to NaCl (540 mM NaCl) and Ca!+ ions are necessary. The receptor whose isolation is described in this report, is involved in secondary aggre-gation processes, which are initiated by a soluble aggregation factor. The primary aggregation of the cells is not influenced by the receptor. Time-course studies with receptor-depleted cells revealed that new aggregation receptor molecules are formed during the aggregation process. By competition experiments it could be shown that high concentrations of soluble aggre-gation receptor molecules inhibit secondary aggregation. The soluble receptor molecules can compete with surface-bound receptor molecules only if these are not linked with the aggre-gation factor. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.614.5401; http://jcs.biologists.org/content/21/2/227.full.pdf |
| الاتاحة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.614.5401 http://jcs.biologists.org/content/21/2/227.full.pdf |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.131DC0BB |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |