Academic Journal
Human mesenchymal stem cells make cardiac connexins and form functional gap junctions
| العنوان: | Human mesenchymal stem cells make cardiac connexins and form functional gap junctions |
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| المؤلفون: | Valiunas, Virginijus, Doronin, Sergey, Valiuniene, Laima, Potapova, Irina, Zuckerman, Joan, Walcott, Benjamin, Robinson, Richard B., Rosen, Michael R., Brink, Peter R., Cohen, Ira S. |
| المصدر: | The Journal of Physiology ; volume 555, issue 3, page 617-626 ; ISSN 0022-3751 1469-7793 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2004 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Human mesenchymal stem cells (hMSCs) are a multipotent cell population with the potential to be a cellular repair or delivery system provided that they communicate with target cells such as cardiac myocytes via gap junctions. Immunostaining revealed typical punctate staining for Cx43 and Cx40 along regions of intimate cell‐to‐cell contact between hMSCs. The staining patterns for Cx45 rather were typified by granular cytoplasmic staining. hMSCs exhibited cell‐to‐cell coupling to each other, to HeLa cells transfected with Cx40, Cx43 and Cx45 and to acutely isolated canine ventricular myocytes. The junctional currents ( I j ) recorded between hMSC pairs exhibited quasi‐symmetrical and asymmetrical voltage ( V j ) dependence. I j records from hMSC–HeLaCx43 and hMSC–HeLaCx40 cell pairs also showed symmetrical and asymmetrical V j dependence, while hMSC–HeLaCx45 pairs always produced asymmetrical I j with pronounced V j gating when the Cx45 side was negative. Symmetrical I j suggests that the dominant functional channel is homotypic, while the asymmetrical I j suggests the activity of another channel type (heterotypic, heteromeric or both). The hMSCs exhibited a spectrum of single channels with transition conductances ( γ j ) of 30–80pS. The macroscopic I j obtained from hMSC–cardiac myocyte cell pairs exhibited asymmetrical V j dependence, while single channel events revealed γ j of the size range 40–100pS. hMSC coupling via gap junctions to other cell types provides the basis for considering them as a therapeutic repair or cellular delivery system to syncytia such as the myocardium. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1113/jphysiol.2003.058719 |
| الاتاحة: | http://dx.doi.org/10.1113/jphysiol.2003.058719 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1113%2Fjphysiol.2003.058719 https://physoc.onlinelibrary.wiley.com/doi/pdf/10.1113/jphysiol.2003.058719 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.1287693B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1113/jphysiol.2003.058719 |
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