Academic Journal

ERH facilitates microRNA maturation through the interaction with the N-terminus of DGCR8

التفاصيل البيبلوغرافية
العنوان: ERH facilitates microRNA maturation through the interaction with the N-terminus of DGCR8
المؤلفون: Kwon, S Chul, Jang, Harim, Shen, Siyuan, Baek, S Chan, Kim, Kijun, Yang, Jihye, Kim, Jeesoo, Kim, Jong-Seo, Wang, Suman, Shi, Yunyu, Li, Fudong, Kim, V Narry
المساهمون: 김종서, 김빛내리, Kim, Jong-Seo, Kim, V Narry
بيانات النشر: Oxford University Press
سنة النشر: 2021
المجموعة: Seoul National University: S-Space
مصطلحات موضوعية: RUDIMENTARY HOMOLOG ERH, MOLECULAR-BASIS, HNRNP A1, ENHANCER, PROTEIN, COMPLEX, DROSHA, CONSERVATION, RECOGNITION, HAIRPINS
الوصف: The microprocessor complex cleaves the primary transcript of microRNA (pri-miRNA) to initiate miRNA maturation. Microprocessor is known to consist of RNase III DROSHA and dsRNA-binding DGCR8. Here, we identify Enhancer of Rudimentary Homolog (ERH) as a new component of Microprocessor. Through a crystal structure and biochemical experiments, we reveal that ERH uses its hydrophobic groove to bind to a conserved region in the N-terminus of DGCR8, in a 2:2 stoichiometry. Knock-down of ERH or deletion of the DGCR8 N-terminus results in a reduced processing of suboptimal pri-miRNAs in polycistronic miRNA clusters. ERH increases the processing of suboptimal pri-miR-451 in a manner dependent on its neighboring pri-miR-144. Thus, the ERH dimer may mediate 'cluster assistance' in which Microprocessor is loaded onto a poor substrate with help from a high-affinity substrate in the same cluster. Our study reveals a role of ERH in the miRNA biogenesis pathway. ; Y ; 1
نوع الوثيقة: article in journal/newspaper
اللغة: unknown
تدمد: 0305-1048
Relation: Nucleic Acids Research, Vol.48 No.19, pp.11097-11112; 121524; https://hdl.handle.net/10371/178034; 000606018400040; 2-s2.0-85095799384
DOI: 10.1093/nar/gkaa827
الاتاحة: https://hdl.handle.net/10371/178034
https://doi.org/10.1093/nar/gkaa827
رقم الانضمام: edsbas.125DEBD0
قاعدة البيانات: BASE
الوصف
تدمد:03051048
DOI:10.1093/nar/gkaa827