Academic Journal
ERH facilitates microRNA maturation through the interaction with the N-terminus of DGCR8
العنوان: | ERH facilitates microRNA maturation through the interaction with the N-terminus of DGCR8 |
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المؤلفون: | Kwon, S Chul, Jang, Harim, Shen, Siyuan, Baek, S Chan, Kim, Kijun, Yang, Jihye, Kim, Jeesoo, Kim, Jong-Seo, Wang, Suman, Shi, Yunyu, Li, Fudong, Kim, V Narry |
المساهمون: | 김종서, 김빛내리, Kim, Jong-Seo, Kim, V Narry |
بيانات النشر: | Oxford University Press |
سنة النشر: | 2021 |
المجموعة: | Seoul National University: S-Space |
مصطلحات موضوعية: | RUDIMENTARY HOMOLOG ERH, MOLECULAR-BASIS, HNRNP A1, ENHANCER, PROTEIN, COMPLEX, DROSHA, CONSERVATION, RECOGNITION, HAIRPINS |
الوصف: | The microprocessor complex cleaves the primary transcript of microRNA (pri-miRNA) to initiate miRNA maturation. Microprocessor is known to consist of RNase III DROSHA and dsRNA-binding DGCR8. Here, we identify Enhancer of Rudimentary Homolog (ERH) as a new component of Microprocessor. Through a crystal structure and biochemical experiments, we reveal that ERH uses its hydrophobic groove to bind to a conserved region in the N-terminus of DGCR8, in a 2:2 stoichiometry. Knock-down of ERH or deletion of the DGCR8 N-terminus results in a reduced processing of suboptimal pri-miRNAs in polycistronic miRNA clusters. ERH increases the processing of suboptimal pri-miR-451 in a manner dependent on its neighboring pri-miR-144. Thus, the ERH dimer may mediate 'cluster assistance' in which Microprocessor is loaded onto a poor substrate with help from a high-affinity substrate in the same cluster. Our study reveals a role of ERH in the miRNA biogenesis pathway. ; Y ; 1 |
نوع الوثيقة: | article in journal/newspaper |
اللغة: | unknown |
تدمد: | 0305-1048 |
Relation: | Nucleic Acids Research, Vol.48 No.19, pp.11097-11112; 121524; https://hdl.handle.net/10371/178034; 000606018400040; 2-s2.0-85095799384 |
DOI: | 10.1093/nar/gkaa827 |
الاتاحة: | https://hdl.handle.net/10371/178034 https://doi.org/10.1093/nar/gkaa827 |
رقم الانضمام: | edsbas.125DEBD0 |
قاعدة البيانات: | BASE |
تدمد: | 03051048 |
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DOI: | 10.1093/nar/gkaa827 |