Academic Journal
CRAFTing Delivery of Membrane Proteins into Protocells using Nanodiscs
| العنوان: | CRAFTing Delivery of Membrane Proteins into Protocells using Nanodiscs |
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| المؤلفون: | Piotr Stępień, Sylwia Świątek, Manuel Yamil Yusef Robles, Joanna Markiewicz-Mizera, Dhanasekaran Balakrishnan, Satomi Inaba-Inoue, Alex H. De Vries, Konstantinos Beis, Siewert J. Marrink, Jonathan G. Heddle |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Biophysics, Biochemistry, Cell Biology, Molecular Biology, Biotechnology, Space Science, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, successful generative engineering, small unilamellar vesicles, giant unilamellar vesicles, functional artificial cells, employing membrane protein, transported lipid bilayer, allow bespoke delivery, delivering membrane proteins, phosphatidylserine lipid membranes, membrane lipid bilayers, protocells using nanodiscs, membrane proteins, lipid bilayers, lipid nanodiscs, subcellular membranes, guv membranes, delivery system, crafting delivery, wide range, useful tools, transient increase |
| الوصف: | For the successful generative engineering of functional artificial cells, a convenient and controllable means of delivering membrane proteins into membrane lipid bilayers is necessary. Here we report a delivery system that achieves this by employing membrane protein-carrying nanodiscs and the calcium-dependent fusion of phosphatidylserine lipid membranes. We show that lipid nanodiscs can fuse a transported lipid bilayer with the lipid bilayers of small unilamellar vesicles (SUVs) or giant unilamellar vesicles (GUVs) while avoiding recipient vesicles aggregation. This is triggered by a simple, transient increase in calcium concentration, which results in efficient and rapid fusion in a one-pot reaction. Furthermore, nanodiscs can be loaded with membrane proteins that can be delivered into target SUV or GUV membranes in a detergent-independent fashion while retaining their functionality. Nanodiscs have a proven ability to carry a wide range of membrane proteins, control their oligomeric state, and are highly adaptable. Given this, our approach may be the basis for the development of useful tools that will allow bespoke delivery of membrane proteins to protocells, equipping them with the cell-like ability to exchange material across outer/subcellular membranes. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/journal_contribution/CRAFTing_Delivery_of_Membrane_Proteins_into_Protocells_using_Nanodiscs/24653344 |
| DOI: | 10.1021/acsami.3c11894.s001 |
| الاتاحة: | https://doi.org/10.1021/acsami.3c11894.s001 https://figshare.com/articles/journal_contribution/CRAFTing_Delivery_of_Membrane_Proteins_into_Protocells_using_Nanodiscs/24653344 |
| Rights: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.11C1F933 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acsami.3c11894.s001 |
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