Academic Journal
IFN-γ stimulates CpG-induced IL-10 production in Bcells via p38 and JNK signalling pathways
| العنوان: | IFN-γ stimulates CpG-induced IL-10 production in Bcells via p38 and JNK signalling pathways |
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| المؤلفون: | Imbrechts, Maya, De Samblancx, Karen, Fierens, Karlien, Brisse, Ellen, Vandenhaute, Jessica, Mitera, Tania, Libert, Claude, Smets, Ide, Goris, An, Wouters, Carine, Matthys, Patrick |
| المصدر: | EUROPEAN JOURNAL OF IMMUNOLOGY ; ISSN: 0014-2980 ; ISSN: 1521-4141 |
| سنة النشر: | 2018 |
| المجموعة: | Ghent University Academic Bibliography |
| مصطلحات موضوعية: | Biology and Life Sciences, Medicine and Health Sciences, REGULATORY B-CELLS, TOLL-LIKE RECEPTORS, PLASMACYTOID DENDRITIC CELLS, COLLAGEN-INDUCED ARTHRITIS, PROTEIN-KINASE INHIBITORS, CD8(+) T-CELLS, INTERFERON-GAMMA, INTERLEUKIN-10 PRODUCTION, AUTOIMMUNE-DISEASES, VACCINE FAILURE, B cells, interleukin-10, mitogen-activated protein, kinases, Toll-like receptor 9 |
| الوصف: | The production of IL-10, a potent immunosuppressive cytokine, must be strictly regulated to ensure a balanced immune response. IFN-, a key cytokine in multiple immune processes and pathologies, is known as an inhibitor of IL-10 production by monocytes and macrophages, but also has some regulatory functions. In the present study, we explored the role of IFN- on Toll-like receptor (TLR)-induced IL-10 production in murine peritoneal and spleen cells and in human peripheral blood mononuclear cells. IFN- inhibited IL-10 production induced by TLR2, TLR3, TLR4 and TLR7/8 agonists, but stimulated IL-10 production when cells were triggered with CpG oligodeoxynucleotides, a specific TLR9 agonist. The stimulatory effect of IFN- on TLR9-induced IL-10 was restricted to Bcells. In line with the increased IL-10, Bcells stimulated with CpG and IFN- profoundly inhibited CD4 T cell proliferation. Further research into the mechanisms involved, revealed that the mitogen-activated protein kinases p38 and JNK are essential players in this stimulatory effect, and that the phosphatase MKP1 - an inhibitor of p38 and JNK activity - is downregulated after combined stimulation with IFN- and CpG. Our data may represent a novel immunoregulatory role of IFN- in Bcells after triggering of TLR9, by stimulating IL-10 production. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://biblio.ugent.be/publication/8583000; http://hdl.handle.net/1854/LU-8583000; http://dx.doi.org/10.1002/eji.201847578; https://biblio.ugent.be/publication/8583000/file/8583001 |
| DOI: | 10.1002/eji.201847578 |
| الاتاحة: | https://biblio.ugent.be/publication/8583000 http://hdl.handle.net/1854/LU-8583000 https://doi.org/10.1002/eji.201847578 https://biblio.ugent.be/publication/8583000/file/8583001 |
| Rights: | No license (in copyright) ; info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.11816BDB |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/eji.201847578 |
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