Academic Journal
Granulocyte colony-stimulating factor alters the systemic metabolomic profile in healthy donors
| العنوان: | Granulocyte colony-stimulating factor alters the systemic metabolomic profile in healthy donors |
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| المؤلفون: | Hatfield, Kimberley Joanne, Melve, Guro Kristin, Bruserud, Øystein |
| المصدر: | 2 ; Metabolomics ; 13 |
| بيانات النشر: | Springer |
| سنة النشر: | 2017 |
| المجموعة: | University of Bergen: Bergen Open Research Archive (BORA-UiB) |
| الوصف: | Introduction: Peripheral blood stem cells mobilized by granulocyte colony-stimulating factor (G-CSF) from healthy donors are commonly used for allogeneic stem cell transplantation. The effect of G-CSF administration on global serum metabolite profiles has not been investigated before. Objectives: This study aims to examine the systemic metabolomic profiles prior to and following administration of G-CSF in healthy adults. Methods: Blood samples were collected from 15 healthy stem cell donors prior to and after administration of G-CSF 10 µg/kg/day for 4 days. Using a non-targeted metabolomics approach, metabolite levels in serum were determined using ultrahigh performance liquid chromatography-tandem mass spectrometry and gas chromatography/mass spectrometry. Results: Comparison of the metabolite profiles of donors before and after G-CSF treatment revealed 239 metabolites that were significantly altered. The major changes of the metabolite profiles following G-CSF administration included alteration of several fatty acids, including increased levels of several medium and long-chain fatty acids, as well as polyunsaturated fatty acids; while there were lower levels of other lipid metabolites such as phospholipids, lysolipids, sphingolipids. Furthermore, there were significantly lower levels of several amino acids and/or their metabolites, including several amino acids with known immunoregulatory functions (methionine, tryptophan, valine). Lastly, the levels of several nucleotides and nucleotide metabolites (guanosine, adenosine, inosine) were also decreased after G-CSF administration, while methylated products were increased. Some of these altered products/metabolites may potentially have angioregulatory effects whereas others may suggest altered intracellular epigenetic regulation. Conclusion: Our results show that G-CSF treatment alters biochemical serum profiles, in particular amino acid, lipid and nucleotide metabolism. Additional studies are needed to further evaluate the relevance of these changes in healthy ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1573-3882 |
| Relation: | urn:issn:1573-3882; https://hdl.handle.net/11250/2727413; https://doi.org/10.1007/s11306-016-1139-x; cristin:1469591 |
| DOI: | 10.1007/s11306-016-1139-x |
| الاتاحة: | https://hdl.handle.net/11250/2727413 https://doi.org/10.1007/s11306-016-1139-x |
| Rights: | Navngivelse 4.0 Internasjonal ; http://creativecommons.org/licenses/by/4.0/deed.no ; Copyright 2016 The Author(s) . |
| رقم الانضمام: | edsbas.116EAF20 |
| قاعدة البيانات: | BASE |
| تدمد: | 15733882 |
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| DOI: | 10.1007/s11306-016-1139-x |