Academic Journal
Centrophilic retrotransposon integration via CENH3 chromatin in Arabidopsis.
| العنوان: | Centrophilic retrotransposon integration via CENH3 chromatin in Arabidopsis. |
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| المؤلفون: | Tsukahara, Sayuri, Bousios, Alexandros, Perez-Roman, Estela, Yamaguchi, Sota, Leduque, Basile, Nakano, Aimi, Naish, Matthew, Osakabe, Akihisa, Toyoda, Atsushi, Ito, Hidetaka, Edera, Alejandro, Tominaga, Sayaka, Juliarni, Kato, Kae, Oda, Shoko, Inagaki, Soichi, Lorković, Zdravko, Nagaki, Kiyotaka, Berger, Frédéric, Kawabe, Akira, Quadrana, Leandro, Henderson, Ian, Kakutani, Tetsuji |
| بيانات النشر: | Springer Science and Business Media LLC //doi.org/10.1038/s41586-024-08319-7 Nature |
| سنة النشر: | 2025 |
| المجموعة: | Apollo - University of Cambridge Repository |
| مصطلحات موضوعية: | Arabidopsis, Centromere, Retroelements, Chromatin, Histones, Arabidopsis Proteins, Terminal Repeat Sequences, Tandem Repeat Sequences, Evolution, Molecular |
| الوصف: | Acknowledgements: We thank T. Sasaki for helpful advice and providing pPLV01-pRPS5a vector, A. Terui for technical assistance, and NASC/ABRC for distributing the seeds. The computations were partially performed on the NIG (National Institute of Genetics) supercomputer at NIG, Japan. This work was supported by grants from Human Frontier Science Program (HFSP) (RGP0025/2021) to I.H. and T.K., BBSRC (BB/V003984/1) to I.H., Japan Science and Technology Agency (JST) CREST (JPMJCR15O1) to T.K., PREST (JPMJPR20K3) to A.O., Japan Society for the Promotion of Science (JSPS) (22KJ0502 to S. Tsukahara, 22H04925 to A.T., 21K06284 to A.K., 21H04977 and 23H00365 to T.K.), Royal Society awards (UF160222, RF/ERE/221032, URF/R/221024, RGF/R1/180006, RGF/EA/201030 and RF/ERE/210069) to A.B. and Centre National de la Recherche Scientifique (IRP SYNERTE) to L.Q. ; In organisms ranging from vertebrates to plants, major components of centromeres are rapidly evolving repeat sequences, such as tandem repeats (TRs) and transposable elements (TEs), which harbour centromere-specific histone H3 (CENH3)1,2. Complete centromere structures recently determined in human and Arabidopsis suggest frequent integration and purging of retrotransposons within the TR regions of centromeres3-5. Despite the high impact of 'centrophilic' retrotransposons on the paradox of rapid centromere evolution, the mechanisms involved in centromere targeting remain poorly understood in any organism. Here we show that both Ty3 and Ty1 long terminal repeat retrotransposons rapidly turnover within the centromeric TRs of Arabidopsis species. We demonstrate that the Ty1/Copia element Tal1 (Transposon of Arabidopsis lyrata 1) integrates de novo into regions occupied by CENH3 in Arabidopsis thaliana, and that ectopic expansion of the CENH3 region results in spread of Tal1 integration regions. The integration spectra of chimeric TEs reveal the key structural variations responsible for contrasting chromatin-targeting specificities to centromeres versus gene-rich regions, ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf; application/zip; text/xml |
| اللغة: | English |
| Relation: | https://www.repository.cam.ac.uk/handle/1810/378785 |
| الاتاحة: | https://www.repository.cam.ac.uk/handle/1810/378785 |
| رقم الانضمام: | edsbas.11512594 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |