Academic Journal
Pumpkin seed oil-loaded chitosan nanoparticles enhance growth, digestive enzymes, and immune response in whiteleg shrimp (Litopenaeus vannamei): Impacts on histopathology and survival against Vibrio parahaemolyticus
| العنوان: | Pumpkin seed oil-loaded chitosan nanoparticles enhance growth, digestive enzymes, and immune response in whiteleg shrimp (Litopenaeus vannamei): Impacts on histopathology and survival against Vibrio parahaemolyticus |
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| المؤلفون: | El-Sayed Hemdan Eissa, Wesam Mohamed Salama, Samia Elbahnaswy, Mai A.M. El-Son, Zienab E. Eldin, Sara Amer, Layla A. Almutairi, Fatma Mohamed Ameen Khalil, Safia M.A. Bahshwan, Mada M. AL-Qurashi, Yasmin M. Abd El-Aziz, Moaheda E.H. Eissa, Aiman A. Alsaegh, Roshmon Thomas Mathew, El Sayed Taha Rizk |
| المصدر: | Aquaculture Reports, Vol 40, Iss , Pp 102599- (2025) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2025 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Pumpkin seed oil-loaded chitosan nanoparticles, Growth promoter, Immunostimulatory, Histopathology, Vibrio parahaemolyticus, Aquaculture. Fisheries. Angling, SH1-691 |
| الوصف: | The research evaluated the innovative effects of pumpkin seed oil-loaded chitosan nanoparticles (ChNPs/PSO) on the growth, body composition, digestive enzymes, immune response, organ histopathology, and survivability of whiteleg shrimp (Litopenaeus vannamei) juveniles against Vibrio parahaemolyticus. Shrimp were fed basal diets (control diets and 1 g of free nano chitosan; ChNPs) supplemented with ChNPs and different levels of ChNPs/PSO at 1 (ChNPs+PSO1), 1.5 (ChNPs+PSO1.5), and 2 (ChNPs+PSO2) mL/kg for a continuous 60 days period. At the end of the feeding trial, shrimp juveniles were exposed to V. parahaemolyticus. The results showed that dietary inclusion of ChNPs/PSO (1.5 or 2 mL) significantly improved the final weight, feed conversion ratio, weight gain, and specific growth rate of shrimp compared to other groups (P < 0.05). Shrimp fed diets supplemented with 1.5 or 2 mL of ChNPs/PSO demonstrated significantly higher survival rates compared to other groups. These groups also exhibited significant increases in crude protein, ash content, phagocytic index, superoxide dismutase, and lipase levels (P < 0.05). The ChNPs+PSO2 group showed the highest amylase activity (P < 0.05). Furthermore, shrimp in these groups displayed significantly elevated total hemocyte counts, lysozyme activity, and bactericidal capacity (P < 0.05). Besides, a significant increase in phenoloxidase and phagocytic activities was observed in the ChNPs+PSO1 group (P < 0.05). The expression levels of prophenoloxidase (LvproPO) and hemocyanin (LvHc) genes were significantly increased in the hepatopancreas of shrimp fed diets enriched with 1.5 or 2 mL of ChNPs/PSO. The survival rate after infection with V. parahaemolyticus was higher in all ChNPs/PSO-enriched groups compared to the control group. Dietary supplementation with ChNPs/PSO restored the histological damages in the muscle, hepatopancreas, and intestine induced by V. parahaemolyticus infection. In summary, pumpkin seed oil-loaded chitosan nanoparticles (ChNPs/PSO) hold ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2352513424006872; https://doaj.org/toc/2352-5134; https://doaj.org/article/86af0abcf0e049e9b6fd65b33d706c73 |
| DOI: | 10.1016/j.aqrep.2024.102599 |
| الاتاحة: | https://doi.org/10.1016/j.aqrep.2024.102599 https://doaj.org/article/86af0abcf0e049e9b6fd65b33d706c73 |
| رقم الانضمام: | edsbas.112E5BF1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.aqrep.2024.102599 |
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