Academic Journal
Laboratory validation of a lateral flow device for the detection of CyHV-3 antigens in gill swabs
| العنوان: | Laboratory validation of a lateral flow device for the detection of CyHV-3 antigens in gill swabs |
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| المؤلفون: | Vrancken, R, Boutier, M, Ronsmans, M, Reschner, A, Leclipteux, T, Lieffrig, F, Collard, A, Mélard, C, Wera, S, Neyts, Johan, Goris, N, Vanderplasschen, A |
| بيانات النشر: | Elsevier/North-Holland Biomedical Press |
| سنة النشر: | 2013 |
| المجموعة: | KU Leuven: Lirias |
| الوصف: | Cyprinid herpesvirus-3 (CyHV-3) induces the highly contagious koi herpesvirus disease (KHVD) and may result in significant economic losses to the ornamental and food-producing carp industry. Suspicion of KHVD is triggered by clinical signs and confirmed using laboratory techniques. The latter are labour- and time-consuming, require specialised equipment and trained personnel. For rapid, on-site detection of CyHV-3, a lateral flow device (LFD) was developed using two monoclonal antibodies directed towards the viral glycoprotein ORF65. The LFD was highly specific with analytical and diagnostic specificities of 100%. Analytical sensitivity ranged between 1.25×10(2) and 2.40×10(4) plaque forming units per ml for isolates originating from geographically distinct regions. In experimentally infected carp, CyHV-3 was detected as early as 4-5 days post infection. Diagnostic sensitivities of 52.6% and 72.2% relative to PCR were recorded, depending on the viral isolate used. When onset of mortality was taken as reference, diagnostic sensitivities increased to 67.0% and 93.3%. The diagnostic sensitivity for freshly found-dead animals was 100%, irrespective of the virus isolate used. Given the high specificity and ease-of-use for on-site detection of CyHV-3, the LFD was regarded fit for purpose as a first-line diagnostic tool for the identification of acute CyHV-3 infections in KHVD affected (koi) carp. ; status: published |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0166-0934 1879-0984 |
| Relation: | Journal of Virological Methods vol:193 issue:2 pages:679-682; https://lirias.kuleuven.be/handle/123456789/413648; http://linkinghub.elsevier.com/retrieve/pii/S0166-0934(13)00302-9; https://lirias.kuleuven.be/bitstream/123456789/413648/2//2013083.pdf |
| الاتاحة: | https://lirias.kuleuven.be/handle/123456789/413648 http://linkinghub.elsevier.com/retrieve/pii/S0166-0934(13)00302-9 https://lirias.kuleuven.be/bitstream/123456789/413648/2//2013083.pdf |
| رقم الانضمام: | edsbas.111130DA |
| قاعدة البيانات: | BASE |
| تدمد: | 01660934 18790984 |
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