التفاصيل البيبلوغرافية
| العنوان: |
Complement component C3 and complement factor B promote growth of cutaneous squamous cell carcinoma |
| المؤلفون: |
Riihilä, P. (Pilvi), Nissinen, L. (Liisa), Farshchian, M. (Mehdi), Kallajoki, M. (Markku), Kivisaari, A. (Atte), Meri, S. (Seppo), Grénman, R. (Reidar), Peltonen, S. (Sirkku), Peltonen, J. (Juha), Pihlajaniemi, T. (Taina), Heljasvaara, R. (Ritva), Kähäri, V.-M. (Veli-Matti) |
| بيانات النشر: |
Elsevier |
| سنة النشر: |
2017 |
| المجموعة: |
Jultika - University of Oulu repository / Oulun yliopiston julkaisuarkisto |
| مصطلحات موضوعية: |
activation, alternative pathway, expression, factor-H, factor-I, human keratinocytes, lung-cancer, progression, skin, tumor microenvironment |
| الوصف: |
Cutaneous squamous cell carcinoma (cSCC) is one of the most common metastatic skin cancers with increasing incidence. We examined the roles of complement component C3 and complement factor B (CFB) in the growth of cSCC. Analysis of cSCC cell lines (n = 8) and normal human epidermal keratinocytes (n = 11) with real-time quantitative PCR and Western blotting revealed up-regulation of C3 and CFB expression in cSCC cells. Immunohistochemical staining revealed stronger tumor cell-specific labeling for C3 and CFB in invasive cSCCs (n = 71) and recessive dystrophic epidermolysis bullosa-associated cSCCs (n = 11) than in cSCC in situ (n = 69), actinic keratoses (n = 63), and normal skin (n = 5). Significant up-regulation of C3 and CFB mRNA expression was noted in chemically induced mouse cSCCs, compared to benign papillomas. Knockdown of C3 and CFB expression inhibited migration and proliferation of cSCC cells and resulted in potent inhibition of extracellular signal-regulated kinase 1/2 activation. Knockdown of C3 and CFB markedly inhibited growth of human cSCC xenograft tumors in vivo. These results provide evidence for the roles of C3 and CFB in the development of cSCC and identify them as biomarkers and potential therapeutic targets in this metastatic skin cancer. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pissn/0002-9440; info:eu-repo/semantics/altIdentifier/eissn/1525-2191 |
| الاتاحة: |
http://urn.fi/urn:nbn:fi-fe201708188152 |
| Rights: |
info:eu-repo/semantics/openAccess ; Copyright 2017 © American Society for Investigative Pathology. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0) ; https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: |
edsbas.109876AA |
| قاعدة البيانات: |
BASE |